分析钙离子通过离子导入促进核黄素穿透角膜基质的效果。
Analysis of the effect of calcium ions on promoting the penetrability of riboflavin into the corneal stroma by iontophoresis.
机构信息
Department of Ophthalmology, The Third Medical Center of Chinese, PLA General Hospital, Yongding Road 69, Beijing, 100039, China.
Department of Ophthalmology, The Sixth Medical Center of Chinese, PLA General Hospital, Beijing, 100048, China.
出版信息
Int Ophthalmol. 2024 Feb 16;44(1):89. doi: 10.1007/s10792-024-03031-x.
PURPOSE
To investigate the effect of calcium ions on promoting the penetrability of riboflavin into the corneal stroma by iontophoresis and to analyse the possible mechanism.
METHODS
Forty rabbits were divided into five groups randomly: 0.1% riboflavin-balanced salt solution (BSS) by iontophoresis group, 0.1% riboflavin-saline solution by iontophoresis group, 0.1% riboflavin-zinc gluconate solution by iontophoresis group, 0.1% riboflavin-calcium gluconate solution by iontophoresis group and classical riboflavin instillation after corneal de-epithelialization as the control group. The riboflavin concentrations in corneal stroma were determined and compared by high-performance liquid chromatography (HPLC) after removing epithelium and endothelium.
RESULTS
Iontophoretic delivery of a 0.1% riboflavin-calcium gluconate solution was the closest to the effect of classical de-epithelialization. The other solvents were unsufficient at enhancing the permeability of the riboflavin.
CONCLUSION
Calcium ions can promote the penetrability of riboflavin into the corneal stroma by iontophoresis.
目的
通过离子导入研究钙离子对促进核黄素穿透角膜基质的作用，并分析其可能的机制。
方法
将 40 只兔子随机分为 5 组：离子导入 0.1%核黄素-平衡盐溶液组、离子导入 0.1%核黄素-生理盐水溶液组、离子导入 0.1%核黄素-葡萄糖酸锌溶液组、离子导入 0.1%核黄素-葡萄糖酸钙溶液组和角膜去上皮后经典核黄素滴眼组。通过高效液相色谱（HPLC）检测去上皮和去内皮后角膜基质中的核黄素浓度，并进行比较。
结果
离子导入 0.1%核黄素-葡萄糖酸钙溶液的效果最接近经典去上皮化的效果。其他溶剂在增强核黄素的通透性方面效果不足。
结论
钙离子可以通过离子导入促进核黄素穿透角膜基质。
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