College of Bioresources Chemical and Materials Engineering, National Demonstration Center for Experimental Light Chemistry Engineering Education, Shaanxi University of Science & Technology, Xi'an, Shaanxi 710021, China.
College of Bioresources Chemical and Materials Engineering, National Demonstration Center for Experimental Light Chemistry Engineering Education, Shaanxi University of Science & Technology, Xi'an, Shaanxi 710021, China.
Carbohydr Polym. 2024 Apr 15;330:121812. doi: 10.1016/j.carbpol.2024.121812. Epub 2024 Jan 15.
Biomacromolecules based injectable and self-healing hydrogels possessing high mechanical properties have widespread potential in biomedical field. However, dynamic features are usually inversely proportional to toughness. It is challenging to simultaneously endow these properties to the dynamic hydrogels. Here, we fabricated an injectable nanocomposite hydrogel (CS-NPs@OSA-l-Gtn) stimultaneously possessing excellent autonomous self-healing performance and high mechanical strength by doping chitosan nanoparticles (CS-NPs) into dynamic polymer networks of oxidized sodium alginate (OSA) and gelatin (Gtn) in the presence of borax. The synergistic effect of the multiple reversible interactions combining dynamic covalent bonds (i.e., imine bond and borate ester bond) and noncovalent interactions (i.e., electrostatic interaction and hydrogen bond) provide effective energy dissipation to endure high fatigue resistance and cyclic loading. The dynamic hydrogel exhibited excellent mechanical properties like maximum 2.43 MPa compressive strength, 493.91 % fracture strain, and 89.54 kJ/m toughness. Moreover, the integrated hydrogel after injection and self-healing could withstand 150 successive compressive cycles. Besides, the bovine serum albumin embedded in CS-NPs could be sustainably released from the nanocomposite hydrogel for 12 days. This study proposes a novel strategy to synthesize an injectable and self-healing hydrogel combined with excellent mechanical properties for designing high-strength natural carriers with sustained protein delivery.
具有高机械性能的基于生物大分子的可注射和自修复水凝胶在生物医学领域具有广泛的应用潜力。然而，动态特性通常与韧性成反比。同时赋予这些特性到动态水凝胶是具有挑战性的。在这里，我们通过在硼砂存在下将壳聚糖纳米粒子 (CS-NPs) 掺杂到氧化的海藻酸钠 (OSA) 和明胶 (Gtn) 的动态聚合物网络中，制备了一种同时具有出色的自主自修复性能和高机械强度的可注射纳米复合水凝胶 (CS-NPs@OSA-l-Gtn)。多种可逆相互作用的协同效应，结合动态共价键（即亚胺键和硼酸酯键）和非共价相互作用（即静电相互作用和氢键），为承受高耐疲劳性和循环载荷提供了有效的能量耗散。该动态水凝胶具有出色的机械性能，如最大 2.43 MPa 的压缩强度、493.91%的断裂应变和 89.54 kJ/m 的韧性。此外，注入和自修复后的集成水凝胶能够承受 150 次连续的压缩循环。此外，CS-NPs 中嵌入的牛血清白蛋白可以从纳米复合水凝胶中持续释放 12 天。本研究提出了一种新的策略，用于合成具有优异机械性能的可注射和自修复水凝胶，用于设计具有持续蛋白输送能力的高强度天然载体。
