Neamah Ghusoon Abdul Kareem, Alkhfaji Muna Abdul Sahib, Shaheed Heba Saleh
Department of Pathology and Poultry Diseases, College of Veterinary Medicine, Al-Qassim Green University, Babylon, Iraq.
J Adv Vet Anim Res. 2023 Dec 31;10(4):647-653. doi: 10.5455/javar.2023.j720. eCollection 2023 Dec.
The main goal of the study was to find whether pumpkin () extract has any preventive or antioxidant properties against acute uranyl acetate (UA)-induced reproductive cytotoxicity.
Four groups each, including 10 adult male rats, were randomly assigned. (GI): the control group was given 1 ml of purified water orally for 30 days. (GII): Rats were given orally, a single dose of 150 mg/kg b.w. UA (GIII): Rats consumed 40 mg/kg b.w. of pumpkin seed extract (PSE) orally every day for 30 days. (GIV): Rats received a single dose (150 mg/kg b.w.) of UA plus a daily oral dose of PSE (40 mg/kg b.w.) for 30 days. Animal sacrifice was used for oxidative stress and histopathological study.
Showed significantly ( ≥ 0.001) elevated malondialdehyde levels in the GII group (6.19 ± 0.4), while GIII and GIV showed no significant differences. Glutathione peroxidase showed a significant ( ≥ 0.001) decrease (2.55 ± 0.2) in the GII group, while in groups (GIII and GIV), it showed a significant ( ≥ 0.001) increase (4.61 ± 0.16, 4.28 ± 0.032), respectively. The histopathological study for GII groups showed sloughing of epithelial cells lining the seminiferous tubules with a decrease in the number of spermatozoa in some tubules. Many sections revealed hyperplasia of the epithelial cells lining the seminiferous tubules with necrosis. The GIII and GIV groups showed normal histological structures with an increase in spermatogenesis in the testes and epididymis tissues.
We concluded that UA causes oxidative stress and histopathological alterations in the rat reproductive system. Pumpkin extract plays a role in improving the activity of the reproductive system.
本研究的主要目的是探究南瓜（）提取物是否对醋酸铀酰（UA）诱导的急性生殖细胞毒性具有预防或抗氧化特性。
随机分为四组，每组10只成年雄性大鼠。（GI）：对照组口服1毫升纯净水，持续30天。（GII）：大鼠口服单剂量150毫克/千克体重的UA。（GIII）：大鼠每天口服40毫克/千克体重的南瓜籽提取物（PSE），持续30天。（GIV）：大鼠接受单剂量（150毫克/千克体重）的UA，外加每日口服剂量的PSE（40毫克/千克体重），持续30天。通过动物处死进行氧化应激和组织病理学研究。
GII组丙二醛水平显著升高（≥0.001）（6.19±0.4），而GIII组和GIV组无显著差异。谷胱甘肽过氧化物酶在GII组显著降低（≥0.001）（2.55±0.2），而在（GIII组和GIV组）中分别显著升高（≥0.001）（4.61±0.16，4.28±0.032）。GII组的组织病理学研究显示，生精小管内衬上皮细胞脱落，部分小管内精子数量减少。许多切片显示生精小管内衬上皮细胞增生并伴有坏死。GIII组和GIV组显示正常组织结构，睾丸和附睾组织中的精子发生增加。
我们得出结论，UA会导致大鼠生殖系统的氧化应激和组织病理学改变。南瓜提取物在改善生殖系统活性方面发挥作用。
