生长分化因子9（GDF9）基因中G1（c.260 G>A）和G4（c.721 G>A）多态性与绵羊产羔数的关系：一项荟萃分析研究。

The relationship between G1 (c.260 G>A) and G4 (c.721 G>A) polymorphisms in the GDF9 gene and the litter size of sheep: A meta-analysis study.

作者信息

Budiyanto Agung, Hartanto Slamet, Widayanti Rini, Setyawan Erif Maha Nugraha, Wardono Heru Ponco, Gustari Sri

机构信息

Department of Reproduction, Obstetrics and Gynecology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.

National Research and Innovation Agency (BRIN), Jakarta, Indonesia.

出版信息

J Adv Vet Anim Res. 2023 Dec 31;10(4):599-607. doi: 10.5455/javar.2023.j715. eCollection 2023 Dec.

DOI:10.5455/javar.2023.j715

PMID:38370904

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10868705/

Abstract

OBJECTIVE

The results of G1 and G4 polymorphisms as litter-size (LS) markers of ewes remain contradictory. The aim was to evaluate the impact of G1 (c.260 G>A) and G4 (c.721 G>A) polymorphisms on the LS of sheep by synthesizing data from multiple previous studies.

METHODS

Data were extracted from 14 eligible articles. The genotypes of G1 and G4 polymorphisms were homozygous wild-type (WW), heterozygous (WM), and homozygous mutant-type (MM). The standardized mean difference (SMD) method using random effect models was employed to determine the effect size of G1 and G4 polymorphisms on LS under dominant, recessive, additive, and co-dominant genetic models. Heterogeneity was analyzed with the I2 statistic index. Publication bias was depicted with funnel plots and tested by Egger's and Begg's tests.

RESULTS

The study showed that the correlation between G1 polymorphism and LS in sheep was not significant (p > 0.05) under all genetic models. The influence of G4 polymorphism on the LS of sheep was found significantly (p < 0.05) under dominant [SMD = 0.28, I2 = 0% (no heterogeneity)] and co-dominant [SMD = -0.14, I2 = 36% (moderate heterogeneity)] genetic models. The WM genotype of G4 polymorphism increased LS, while the MM genotype reduced LS in sheep. Publication bias among G1 and G4 polymorphism studies was absent in all genetic models.

CONCLUSION

Thus, the study revealed that G4 polymorphism could be a potential genetic marker for LS in ewes. On the contrary, G1 polymorphism has no association with the LS of ewes.

摘要

目的

作为母羊产仔数（LS）标记的G1和G4多态性的研究结果仍存在矛盾。本研究旨在通过综合以往多项研究的数据，评估G1（c.260 G>A）和G4（c.721 G>A）多态性对绵羊产仔数的影响。

方法

从14篇符合条件的文章中提取数据。G1和G4多态性的基因型为纯合野生型（WW）、杂合型（WM）和纯合突变型（MM）。采用随机效应模型的标准化均值差（SMD）方法，确定G1和G4多态性在显性、隐性、加性和共显性遗传模型下对产仔数的效应大小。用I2统计指数分析异质性。用漏斗图描述发表偏倚，并用Egger检验和Begg检验进行检验。

结果

研究表明，在所有遗传模型下，G1多态性与绵羊产仔数之间的相关性均不显著（p>0.05）。在显性[SMD = 0.28，I2 = 0%（无异质性）]和共显性[SMD = -0.14，I2 = 36%（中度异质性）]遗传模型下，发现G4多态性对绵羊产仔数有显著影响（p<0.05）。G4多态性的WM基因型增加了绵羊的产仔数，而MM基因型则降低了绵羊的产仔数。在所有遗传模型中，G1和G4多态性研究均不存在发表偏倚。