Thupakula Sreenu, Nimmala Shiva Shankar Reddy, Dawood Shauq Mumtaz, Padiya Raju
Department of Biochemistry, University College of Science, Osmania University, Amberpet, Hyderabad, Telangana State 500007 India.
3 Biotech. 2024 Mar;14(3):76. doi: 10.1007/s13205-024-03929-4. Epub 2024 Feb 14.
Diabetes is often associated with increased oxidative stress caused by an imbalance between detoxification and ROS production. Unfortunately, many commercial drugs available today for treating this disease have adverse side effects and ultimately fail to restore glucose homeostasis. Therefore, finding a dietary anti-diabetic remedy that is safe, effective, and economical is crucial. In this study, GC-MS analysis, subsequent HPLC-assisted fractionation, and SPE-based purification led to identifying and purifying of key components such as phloroglucinol and total procyanidin dimer (procyanidin dimer and procyanidin dimer gallate) from methanolic seed extract of . In-vitro anti-diabetic screening of various fractions derived from methanolic extract along with individual components and their combinations revealed the potential synergistic behaviour of phloroglucinol and total procyanidin dimer with the lowest IC50 of 48.21 ± 3.54 µg/mL for α-glucosidase and 63.06 ± 5.38 µg/mL for α-amylase inhibition which is found to be superior to the effect shown by the standard Epigallocatechin gallate. Later Glucose utilization studies demonstrated the concentration-dependent effect of Phloroglucinol and total procyanidin dimer, and that has raised the glucose uptake by approximately 36-57% in HepG2 cells and 35-58% in L6 myocytes over a concentration of 50-100 µg/mL. The superior anti-diabetic effect of Phloroglucinol and total procyanidin dimer was proved by the suppression of oxidative stress with an IC50 of 7.92 ± 0.36 µg/mL for DPPH scavenging and 16.87 ± 1.24 µg/mL for SOD scavenging which is competent with the standard ascorbic acid. According to this study, suppressing ROS levels by phloroglucinol and total procyanidin dimer would be the underlying mechanism for the synergistic anti-diabetic effect of this combination.
The online version contains supplementary material available at 10.1007/s13205-024-03929-4.
糖尿病通常与解毒和活性氧生成失衡导致的氧化应激增加有关。不幸的是，如今许多用于治疗该疾病的商业药物都有不良副作用，最终无法恢复葡萄糖稳态。因此，找到一种安全、有效且经济的饮食抗糖尿病疗法至关重要。在本研究中，通过气相色谱 - 质谱分析、随后的高效液相色谱辅助分级分离以及基于固相萃取的纯化，从[植物名称]的甲醇种子提取物中鉴定并纯化出了关键成分，如间苯三酚和原花青素二聚体总量（原花青素二聚体和原花青素二聚体没食子酸酯）。对甲醇提取物衍生的各种馏分以及单个成分及其组合进行的体外抗糖尿病筛选显示，间苯三酚和原花青素二聚体总量具有潜在的协同作用，对α - 葡萄糖苷酶的最低半数抑制浓度（IC50）为48.21±3.54μg/mL，对α - 淀粉酶抑制的IC50为63.06±5.38μg/mL，发现其效果优于标准表没食子儿茶素没食子酸酯。随后的葡萄糖利用研究证明了间苯三酚和原花青素二聚体总量的浓度依赖性效应，在50 - 100μg/mL的浓度范围内，其在HepG2细胞中使葡萄糖摄取增加了约36 - 57%，在L6肌细胞中增加了35 - 58%。间苯三酚和原花青素二聚体总量对二苯基苦味酰基自由基（DPPH）清除的IC50为7.92±0.36μg/mL，对超氧化物歧化酶（SOD）清除的IC50为16.87±1.24μg/mL，与标准抗坏血酸相当，通过抑制氧化应激证明了其卓越的抗糖尿病效果。根据本研究，间苯三酚和原花青素二聚体总量抑制活性氧水平将是该组合协同抗糖尿病作用的潜在机制。
在线版本包含可在10.1007/s13205 - 024 - 03929 - 4获取的补充材料。
