Qufu Normal University, Qufu 273165, China.
Qufu Normal University, Qufu 273165, China; School of Applied Science, Temasek Polytechnic, 529757, Singapore.
Int J Biol Macromol. 2024 Apr;263(Pt 1):130275. doi: 10.1016/j.ijbiomac.2024.130275. Epub 2024 Feb 17.
Polysaccharides from natural products play multiple roles and have extensive bioactivities in life process. Bioactivities of polysaccharides (e.g., Lentinan, Schizophyllan, Scleroglucan, Curdlan, Cinerean) have a close relation to their chain conformation. Compared to other types of polysaccharides, the conformation of β-glucan has been studied more. The major research methods of conformation of polysaccharides from natural products (Congo red experiment, circular dichroism spectrum, viscosity method, light scattering method, size exclusion chromatography, atomic force microscope), corresponding experimental schemes, and the external factors affecting polysaccharide conformation were reviewed in this paper. These research methods of conformation have been widely used, among which Congo red experiment and viscosity method are the most convenient ones to study the morphological changes of polysaccharide chains.
天然产物多糖在生命过程中发挥多种作用，并具有广泛的生物活性。多糖的生物活性（例如，香菇多糖、裂褶多糖、葡聚糖、鹿角菜胶、云芝多糖）与其链构象密切相关。与其他类型的多糖相比，β-葡聚糖的构象已经得到了更深入的研究。本文综述了天然产物多糖构象的主要研究方法（刚果红实验、圆二色光谱、粘度法、光散射法、尺寸排阻色谱、原子力显微镜）、相应的实验方案以及影响多糖构象的外部因素。这些构象研究方法已被广泛应用，其中刚果红实验和粘度法是研究多糖链形态变化最方便的方法。
