Ingram D L, Collier A M, Pendergrass E, King S H
J Clin Microbiol. 1979 May;9(5):570-4. doi: 10.1128/jcm.9.5.570-574.1979.
Nasopharyngeal isolates of H. influenzae were typed by the slide agglutination test, the Quelling reaction, the latex agglutination test, countercurrent immunoelectrophoresis, and the antiserum agar test. These tests gave essentially comparable results, with countercurrent immunoelectrophoresis and latex agglutination being slightly more sensitive. Cross-reactive problems encountered with latex agglutination and the expense of performing countercurrent immunoelectrophoresis or the antiserum agar test made these tests less practical than the slide agglutination test to identify single strains that were already isolated. The Quellung reaction and slide agglutination were the most rapid tests used to type an organism. For mass screening of multiple samples, countercurrent immunoelectrophoresis was the simplest technique. The antiserum agar test was slow but was the best technique to screen nasopharyngeal swab cultures to identify the presence of any encapsulated strains in the mixed flora. Whether any of the above techniques were as sensitive as the immunofluorescence test was not evaluated in this study.
通过玻片凝集试验、荚膜肿胀反应、乳胶凝集试验、对流免疫电泳和抗血清琼脂试验对流感嗜血杆菌的鼻咽分离株进行分型。这些试验得出的结果基本相当，其中对流免疫电泳和乳胶凝集试验的灵敏度略高。乳胶凝集试验中遇到的交叉反应问题以及进行对流免疫电泳或抗血清琼脂试验的费用，使得这些试验在鉴定已分离的单一菌株时不如玻片凝集试验实用。荚膜肿胀反应和玻片凝集试验是用于对生物体进行分型的最快试验。对于多个样本的大规模筛查，对流免疫电泳是最简单的技术。抗血清琼脂试验速度较慢，但却是筛查鼻咽拭子培养物以确定混合菌群中是否存在任何包膜菌株的最佳技术。本研究未评估上述任何技术是否与免疫荧光试验一样灵敏。
