Himmelreich C A, Barenkamp S J, Storch G A
J Clin Microbiol. 1985 Feb;21(2):158-60. doi: 10.1128/jcm.21.2.158-160.1985.
This study compared four methods for serotyping isolates of Haemophilus influenzae. Slide agglutination with commercial antisera (Difco Laboratories and Wellcome Diagnostics), coagglutination (Phadebact Haemophilus Test [Pharmacia Fine Chemicals]), latex agglutination with affinity-purified anticapsular antibody, and counterimmunoelectrophoresis with multiple antisera were used to serotype 80 isolates of H. influenzae. Coagglutination and counterimmunoelectrophoresis correctly identified all 80 isolates as either type b or not type b. Slide agglutination and latex agglutination each successfully identified 76 of the 80 isolates; however, each of these two methods failed to type four isolates because of agglutination of controls. We recommend slide agglutination or coagglutination as the serotyping methods of choice in most laboratories because they are simple, accurate, and rapid. Slide agglutination with Difco antiserum can be performed at the lowest cost.
本研究比较了四种对流感嗜血杆菌分离株进行血清分型的方法。使用商业抗血清（Difco实验室和威康诊断公司）进行玻片凝集、协同凝集（Phadebact流感嗜血杆菌试验[法玛西亚精细化工公司]）、用亲和纯化的抗荚膜抗体进行乳胶凝集以及用多种抗血清进行对流免疫电泳，对80株流感嗜血杆菌分离株进行血清分型。协同凝集和对流免疫电泳正确地将所有80株分离株鉴定为b型或非b型。玻片凝集和乳胶凝集分别成功鉴定出80株分离株中的76株；然而，这两种方法中的每一种都因对照出现凝集而未能对4株分离株进行分型。我们推荐在大多数实验室将玻片凝集或协同凝集作为血清分型的首选方法，因为它们简单、准确且快速。使用Difco抗血清进行玻片凝集的成本最低。
