Dias J C, Hofer E
Mem Inst Oswaldo Cruz. 1985 Oct-Dec;80(4):411-21. doi: 10.1590/s0074-02761985000400006.
From 154 food samples, including vegetables (lettuce), milk and meals served at school it was possible to isolate and identify 400 Gram negative bacilli distributed among 339 enteric bacteria (Escherichia, Shigella, Citrobacter, Klebsiella, Enterobacter, Serratia and Proteus) and other 61 non enteric bacilli (Acinetobacter, Flavobacterium, Aeromonas and Pseudomonas). Submitting this cultures to the drugs sulfadiazine (Su), streptomycin (Sm), tetracycline (Tc), chloramphenicol (Cm), kanamycin (Km), ampicillin (Ap), nalidixic acid (Nal) and gentamycin (Gm) it was observed only six stocks susceptible to all drugs and total sensibility to Gm. Among enteric bacteria the profiles Su (27,6%) and Su-Ap (39,6%) predominated, while for the non enteric bacilli percentages of 18.0 for Ap and 9.8 for Su-Ap were detected. Aiming to better characterization of resistance, experiments of conjugation were made with standard strains of Escherichia coli K 12. Great concern was raised by the recognition of these cultures due to the elevated R+ taxes for the enteric bacilli that were close to 90% (milk and food at school) and about 70% in relation to lettuce.
从154份食物样本中，包括蔬菜（生菜）、牛奶和学校提供的膳食，共分离并鉴定出400株革兰氏阴性杆菌，它们分布在339株肠道细菌（大肠杆菌、志贺氏菌、柠檬酸杆菌、克雷伯氏菌、肠杆菌、沙雷氏菌和变形杆菌）以及其他61株非肠道杆菌（不动杆菌、黄杆菌、气单胞菌和假单胞菌）中。将这些培养物分别置于磺胺嘧啶（Su）、链霉素（Sm）、四环素（Tc）、氯霉素（Cm）、卡那霉素（Km）、氨苄青霉素（Ap）、萘啶酸（Nal）和庆大霉素（Gm）中，仅观察到6株菌株对所有药物敏感且对庆大霉素完全敏感。在肠道细菌中，磺胺嘧啶（27.6%）和磺胺嘧啶-氨苄青霉素（39.6%）的耐药谱占主导，而对于非肠道杆菌，检测到氨苄青霉素的耐药率为18.0%，磺胺嘧啶-氨苄青霉素的耐药率为9.8%。为了更好地表征耐药性，用大肠杆菌K12标准菌株进行了接合实验。由于肠道杆菌的R+率升高，这些培养物引起了极大关注，在牛奶和学校食物中的R+率接近90%，而生菜中的R+率约为70%。
