Tang Guofeng, Li Zhentao, Ding Chengbin, Zhao Jiang, Xing Xianglong, Sun Yan, Qiu Xiaozhong, Wang Leyu
Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, PR China.
Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangzhou, Guangdong, 510515, PR China.
Bioact Mater. 2024 Feb 14;35:362-381. doi: 10.1016/j.bioactmat.2024.02.012. eCollection 2024 May.
Cell implantation offers an appealing avenue for heart repair after myocardial infarction (MI). Nevertheless, the implanted cells are subjected to the aberrant myocardial niche, which inhibits cell survival and maturation, posing significant challenges to the ultimate therapeutic outcome. The functional cardiac patches (CPs) have been proved to construct an elastic conductive, antioxidative, and angiogenic microenvironment for rectifying the aberrant microenvironment of the infarcted myocardium. More importantly, inducing implanted cardiomyocytes (CMs) adapted to the anisotropic arrangement of myocardial tissue by bioengineered structural cues within CPs are more conducive to MI repair. Herein, a functional Cig/(TA-Cu) CP served as biomimetic cardiac niche was fabricated based on structural anisotropic cigarette filter by modifying with tannic acid (TA)-chelated Cu (TA-Cu complex) via a green method. This CP possessed microstructural anisotropy, electrical conductivity and mechanical properties similar to natural myocardium, which could promote elongation, orientation, maturation, and functionalization of CMs. Besides, the Cig/(TA-Cu) CP could efficiently scavenge reactive oxygen species, reduce CM apoptosis, ultimately facilitating myocardial electrical integration, promoting vascular regeneration and improving cardiac function. Together, our study introduces a functional CP that integrates multimodal cues to create a biomimetic cardiac niche and provides an effective strategy for cardiac repair.
细胞植入为心肌梗死（MI）后的心脏修复提供了一条有吸引力的途径。然而，植入的细胞会受到异常心肌微环境的影响，这会抑制细胞存活和成熟，给最终的治疗效果带来重大挑战。功能性心脏补片（CPs）已被证明能够构建一个弹性导电、抗氧化和促血管生成的微环境，以纠正梗死心肌的异常微环境。更重要的是，通过CPs内的生物工程结构线索诱导植入的心肌细胞（CMs）适应心肌组织的各向异性排列，更有利于心肌梗死的修复。在此，基于结构各向异性的香烟滤嘴，通过绿色方法用单宁酸（TA）螯合的铜（TA-Cu络合物）进行修饰，制备了一种作为仿生心脏微环境的功能性Cig/(TA-Cu) CP。这种CP具有与天然心肌相似的微观结构各向异性、导电性和机械性能，可促进CMs的伸长、定向、成熟和功能化。此外，Cig/(TA-Cu) CP能够有效清除活性氧，减少CMs凋亡，最终促进心肌电整合，促进血管再生并改善心脏功能。总之，我们的研究引入了一种整合多模态线索以创建仿生心脏微环境的功能性CP，并为心脏修复提供了一种有效的策略。
