Badrillah Nadhirah, Susanti Deny, Kamil Tengku Karmila Tengku Mohd, Swandiny Greesty Finotory, Widyastuti Yuli, Zaini Erizal, Taher Muhammad
Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, 25200, Kuantan, Pahang, Malaysia.
Department of Chemistry, Faculty of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, 25200, Kuantan, Pahang, Malaysia.
Heliyon. 2024 Feb 12;10(4):e25454. doi: 10.1016/j.heliyon.2024.e25454. eCollection 2024 Feb 29.
Silver nanoparticle is widely used in various field including medical, cosmetic, food and industrial purposes due to their unique properties in electrical conductivity, thermal, and biological activities. In the medical field, silver nanoparticles (AgNPs) have been reported to have strong antimicrobial and cytotoxic activities. This study aimed to synthesize and characterize silver nanoparticles (AgNPs) using (MP) extract and to evaluate the antimicrobial and cytotoxic activities of the synthesised MP-AgNPs. Green method of Ultrasound Assisted Extraction (UAE) was used to extract the leaves of . Liquid Chromatography -Mass Spectrometry/Quadrupole time-of-flight (LC-MS/QTOF) was used to identify the compounds in the leaf extract . Characterisation of the synthesised nanoparticles involved ultraviolet-visible (UV-Vis), Fourier Transform Infrared (FTIR), scanning electromagnetic microscopy (SEM), Zeta potential Analyzer and Particle Size Analyzer. The cytotoxic assay was conducted on MCF-7 and Caco-2 cell lines by MTT assay. Antimicrobial activity was tested on Gram-negative and Gram-positive bacteria using the disc diffusion method. Based on LC-MS/QTOF analysis, 430 compounds were found. The identified major compounds consist of amino acids, polyphenols, steroids, terpenoids and heterocyclic compounds which possibly act as reducing agents. 1 mM, 5 mM and 10 mM of silver nitrate solution were mixed with the leaf extract to form silver nanoparticles. 1.2 mg/ml of MP-AgNPs were found to have antibacterial activity against and with inhibitory zones of 8.0 ± 0.36 mm, 8.5 ± 0.45 mm, 7.5 ± 0.36 mm, and 9.0 ± 0.40 mm respectively. MP-AgNPs showed no cytotoxic activity against Caco-2 and MCF-7 cells. In conclusion, the presence of major amine compounds such as 10,11-dihydro-10,11-dihydroxyprotriptyline and harderoporphyrin in the extract facilitated the synthesis of AgNPs and the nanoparticle showed weak bioactivities in the assay conducted.
由于银纳米颗粒在导电性、热性能和生物活性方面具有独特性质，其在包括医学、化妆品、食品和工业用途等各个领域都有广泛应用。在医学领域，据报道银纳米颗粒（AgNPs）具有很强的抗菌和细胞毒性活性。本研究旨在使用（MP）提取物合成并表征银纳米颗粒（AgNPs），并评估合成的MP - AgNPs的抗菌和细胞毒性活性。采用超声辅助提取（UAE）的绿色方法提取的叶子。液相色谱 - 质谱联用/四极杆飞行时间（LC - MS/QTOF）用于鉴定叶提取物中的化合物。合成纳米颗粒的表征涉及紫外可见（UV - Vis）、傅里叶变换红外（FTIR）、扫描电子显微镜（SEM）、Zeta电位分析仪和粒度分析仪。通过MTT法对MCF - 7和Caco - 2细胞系进行细胞毒性测定。使用纸片扩散法对革兰氏阴性菌和革兰氏阳性菌进行抗菌活性测试。基于LC - MS/QTOF分析，发现了430种化合物。鉴定出的主要化合物包括氨基酸、多酚、类固醇、萜类化合物和杂环化合物，它们可能作为还原剂。将1 mM、5 mM和10 mM的硝酸银溶液与叶提取物混合以形成银纳米颗粒。发现1.2 mg/ml的MP - AgNPs对和具有抗菌活性，抑菌圈分别为8.0±0.36 mm、8.5±0.45 mm、7.5±0.36 mm和9.0±0.40 mm。MP - AgNPs对Caco - 2和MCF - 7细胞未显示细胞毒性活性。总之，提取物中主要胺类化合物如10,11 - 二氢 - 10,11 - 二羟基普罗替林和硬卟啉的存在促进了AgNPs的合成，并且该纳米颗粒在进行的测定中显示出较弱的生物活性。
