College of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje University, 197 Inje-ro, Gimhae, Gyeongnam 50834, Republic of Korea.
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, 2238 Beijing Road, Kunming, Yunnan Province 650205, China.
Mater Sci Eng C Mater Biol Appl. 2019 Aug;101:204-216. doi: 10.1016/j.msec.2019.03.095. Epub 2019 Mar 26.
We synthesized silver nanoparticles using thirty Chinese plant extracts via a green synthetic strategy. UV-visible spectra showed that the silver nanoparticles have an absorbance at 450 nm. Among the thirty extracts, seven extracts (Cratoxylum formosum, Phoebe lanceolata, Scurrula parasitica, Ceratostigma minus, Mucuna birdwoodiana, Myrsine africana and Lindera strychnifolia) exhibited the successful synthesis of silver nanoparticles. These seven extracts showed higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power than the other extracts. The silver nanoparticles synthesized using these seven extracts were mostly spherical with high colloidal stability. The cytotoxicity of these seven silver nanoparticle samples on human lung cancer cells (A549) was clearly higher than that of the extracts alone. Furthermore, the cytotoxicity was affected by the presence or absence of fetal bovine serum. Moreover, the cytotoxicity of the silver nanoparticles synthesized with Cratoxylum formosum and Mucuna birdwoodiana extracts resulted in apoptotic cell death in A549 cells. The wound healing activity observed by the cell scratch method on mouse fibroblast cells (NIH3T3) suggested that the Lindera strychnifolia extract produced silver nanoparticles with decent activity. These results provide ample and systematic information for researchers on the green synthesis of silver nanoparticles using plant extracts.
我们使用 30 种中国植物提取物通过绿色合成策略合成了银纳米粒子。紫外可见光谱表明，银纳米粒子在 450nm 处有吸收峰。在 30 种提取物中，有 7 种提取物（海南蒲桃、柳叶榕、钩吻、地桃花、三叶鸡血藤、山桐子和黄皮）成功合成了银纳米粒子。这 7 种提取物的 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性和还原能力均高于其他提取物。这 7 种提取物合成的银纳米粒子大多为球形，具有较高的胶体稳定性。这 7 种银纳米粒子样品对人肺癌细胞（A549）的细胞毒性明显高于单独使用提取物的毒性。此外，细胞毒性受胎牛血清的存在与否影响。此外，用海南蒲桃和三叶鸡血藤提取物合成的银纳米粒子导致 A549 细胞发生凋亡性细胞死亡。用细胞划痕法在小鼠成纤维细胞（NIH3T3）上观察到的愈合活性表明，黄皮提取物产生的银纳米粒子具有良好的活性。这些结果为研究人员提供了大量系统的信息，用于使用植物提取物进行银纳米粒子的绿色合成。
