Ilie Cornelia-Ioana, Spoiala Angela, Chircov Cristina, Dolete Georgiana, Oprea Ovidiu-Cristian, Vasile Bogdan-Stefan, Crainiceanu Simona Adriana, Nicoara Adrian-Ionut, Marinas Ioana Cristina, Stan Miruna Silvia, Ditu Lia-Mara, Ficai Anton, Oprea Eliza
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania.
National Centre for Micro and Nanomaterials and National Centre for Food Safety, National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, Romania.
Antioxidants (Basel). 2024 Jul 24;13(8):895. doi: 10.3390/antiox13080895.
The gut microbiota dysbiosis that often occurs in cancer therapy requires more efficient treatment options to be developed. In this concern, the present research approach is to develop drug delivery systems based on magnetite nanoparticles (MNPs) as nanocarriers for bioactive compounds. First, MNPs were synthesized through the spraying-assisted coprecipitation method, followed by loading bee pollen or bee bread extracts and an antitumoral drug (5-fluorouracil/5-FU). The loaded-MNPs were morphologically and structurally characterized through transmission electron microscopy (TEM), selected area electron diffraction (SAED), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Dynamic Light Scattering (DLS), and thermogravimetric analysis. UV-Vis spectroscopy was applied to establish the release profiles and antioxidant activity. Furthermore, the antibacterial and antitumoral activity of loaded-MNPs was assessed. The results demonstrate that MNPs with antioxidant, antibacterial, antiproliferative, and prebiotic properties are obtained. Moreover, the data highlight the improvement of 5-FU antibacterial activity by loading on the MNPs' surface and the synergistic effects between the anticancer drug and phenolic compounds (PCs). In addition, the prolonged release behavior of PCs for many hours (70-75 h) after the release of 5-FU from the developed nanocarriers is an advantage, at least from the point of view of the antioxidant activity of PCs. Considering the enhancement of MF9 growth and antitumoral activity, this study developed promising drug delivery alternatives for colorectal cancer therapy.
癌症治疗中经常出现的肠道微生物群失调需要开发更有效的治疗方案。在这方面，目前的研究方法是开发基于磁铁矿纳米颗粒（MNPs）的药物递送系统，作为生物活性化合物的纳米载体。首先，通过喷雾辅助共沉淀法合成MNPs，然后负载蜂花粉或蜂粮提取物以及一种抗肿瘤药物（5-氟尿嘧啶/5-FU）。通过透射电子显微镜（TEM）、选区电子衍射（SAED）、扫描电子显微镜（SEM）、X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、动态光散射（DLS）和热重分析对负载的MNPs进行形态和结构表征。应用紫外可见光谱来确定释放曲线和抗氧化活性。此外，还评估了负载MNPs的抗菌和抗肿瘤活性。结果表明，获得了具有抗氧化、抗菌、抗增殖和益生元特性的MNPs。此外，数据突出了5-FU负载在MNPs表面后抗菌活性的提高以及抗癌药物与酚类化合物（PCs）之间的协同作用。此外，从PCs的抗氧化活性角度来看，在已开发的纳米载体中5-FU释放后PCs长达数小时（70-75小时）的缓释行为是一个优势。考虑到MF9生长和抗肿瘤活性的增强，本研究为结直肠癌治疗开发了有前景的药物递送替代方案。
