AIT Austrian Institute of Technology GmbH, 3430 Tulln, Austria.
Laboratory for Life Sciences and Technology (LiST), Faculty of Medicine and Dentistry, Danube Private University, 3500 Krems, Austria.
Nanoscale Horiz. 2024 Mar 25;9(4):598-608. doi: 10.1039/d3nh00590a.
We report on the synthesis of "clickable" graphene nanoribbons (GNRs) and their application as a versatile interface for electrochemical biosensors. GNRs are successfully deposited on gold-coated working electrodes and serve as a platform for the covalent anchoring of a bioreceptor (, a DNA aptamer), enabling selective and sensitive detection of Interleukin 6 (IL6). Moreover, when applied as the intermediate linker on reduced graphene oxide (rGO)-based field-effect transistors (FETs), the GNRs provide improved robustness compared to conventional aromatic bi-functional linker molecules. GNRs enable an orthogonal and covalent attachment of a recognition unit with a considerably higher probe density than previously established methods. Interestingly, we demonstrate that GNRs introduce photoluminescence (PL) when applied to rGO-based FETs, paving the way toward the simultaneous optical and electronic probing of the attached biointerface.
我们报告了“点击化学”石墨烯纳米带（GNRs）的合成及其作为电化学生物传感器通用界面的应用。GNRs 成功地沉积在镀金工作电极上，并作为共价固定生物受体（一种 DNA 适体）的平台，实现了对白细胞介素 6（IL6）的选择性和灵敏检测。此外，当作为基于还原氧化石墨烯（rGO）的场效应晶体管（FET）中的中间连接子时，与传统的芳族双官能连接子分子相比，GNRs 提供了更高的稳定性。GNRs 能够实现识别单元的正交和共价连接，其探针密度比以前的方法高得多。有趣的是，我们证明了当 GNRs 应用于基于 rGO 的 FET 时，会产生光致发光（PL），为附着的生物界面的光学和电子同时探测铺平了道路。
