Govindarajan S, Valinluck B
Arch Pathol Lab Med. 1985 May;109(5):398-9.
Hepatitis B-associated delta agent, a defective RNA virus requiring helper functions of hepatitis B virus (HBV), has been shown to interfere with HBV replication. Low titers of serum hepatitis B surface antigen, absence of hepatitis B e antigen, and low levels of stainable hepatitis B core antigen in liver cells usually seen in chronic delta infection are indirect evidences of such an interference. Measurement of serum HBV-DNA by hybridization with phosphorus 32-labeled HBV-clone DNA is the most sensitive method currently available to detect HBV replication. Using this method, we found that only two of 13 patients with chronic delta infection showed serum HBV-DNA positivity in comparison with seven of 14 patients who had chronic hepatitis B without delta infection. These two groups were matched for hepatitis B e antigen status and liver histopathology. Thus, we report direct evidence of delta agent interfering with the replication of the helper (HBV) virus.
乙型肝炎相关丁型肝炎病毒是一种有缺陷的RNA病毒，需要乙型肝炎病毒（HBV）的辅助功能，已被证明会干扰HBV复制。慢性丁型肝炎感染中常见的血清乙型肝炎表面抗原滴度低、乙型肝炎e抗原缺失以及肝细胞中可染色的乙型肝炎核心抗原水平低，都是这种干扰的间接证据。用磷32标记的HBV克隆DNA杂交法检测血清HBV-DNA是目前检测HBV复制最敏感的方法。使用这种方法，我们发现13例慢性丁型肝炎感染患者中只有2例血清HBV-DNA呈阳性，而14例无丁型肝炎感染的慢性乙型肝炎患者中有7例呈阳性。这两组在乙型肝炎e抗原状态和肝脏组织病理学方面相匹配。因此，我们报告了丁型肝炎病毒干扰辅助病毒（HBV）复制的直接证据。
