Michl J, Pieczonka M M, Unkeless J C, Silverstein S C
J Exp Med. 1979 Sep 19;150(3):607-21. doi: 10.1084/jem.150.3.607.
We have examined the Fc- and complement-receptor function of resident and thioglycollate-elicited mouse peritoneal macrophages plated on surfaces coated with rabbit antibody-antigen complexes and with complement. We derive four major conclusions from these studies. (a) The trypsin-resistant Fc receptors of resident and thioglycollate-elicited macrophages are completely modulated when these cells are plated on rabbit antibody-antigen complexes. Residual Fc receptor activity is a result of the incomplete modulation of trypsin-sensitive IgG2a receptors. (b) The complement receptors of thioglycollate-elicited macrophages, but not of resident macrophages, are modulated when these cells are plated on complement-coated surfaces. The capacity of the two cell types to modulate their complement receptors is correlated with their ability to ingest complement-coated erythrocytes. (c) The complement and Fc receptors of both types of macrophages move independently of one another. (d) Complement masks the Fc segments of IgG in immune complexes thereby rendering them ineffective as ligands for macrophage Fc receptors.
我们研究了接种于包被有兔抗体 - 抗原复合物及补体的表面的驻留型和巯基乙酸盐诱导的小鼠腹腔巨噬细胞的Fc受体和补体受体功能。我们从这些研究中得出四个主要结论。(a) 当这些细胞接种于兔抗体 - 抗原复合物上时，驻留型和巯基乙酸盐诱导的巨噬细胞的抗胰蛋白酶Fc受体完全被调节。残余的Fc受体活性是对胰蛋白酶敏感的IgG2a受体未完全被调节的结果。(b) 当巯基乙酸盐诱导的巨噬细胞接种于补体包被的表面时，其补体受体被调节，而驻留型巨噬细胞的补体受体则未被调节。这两种细胞类型调节其补体受体的能力与其摄取补体包被红细胞的能力相关。(c) 两种类型巨噬细胞的补体受体和Fc受体彼此独立移动。(d) 补体掩盖了免疫复合物中IgG的Fc片段，从而使其作为巨噬细胞Fc受体的配体无效。
