Kavruk Murat, Babaie Zahra, Kibar Güneş, Çetin Barbaros, Yeşilkaya Hasan, Amrani Yassine, Dursun Ali Doğan, Özalp V Cengiz
Department of Medical Biology, Faculty of Medicine, İstanbul Aydın University, İstanbul, 34295, Türkiye.
Microfluidics & Lab-on-a-chip Research Group, İ.D. Bilkent University, Ankara, 06800, Türkiye.
Mikrochim Acta. 2024 Apr 23;191(5):285. doi: 10.1007/s00604-024-06322-3.
One significant constraint in the advancement of biosensors is the signal-to-noise ratio, which is adversely affected by the presence of interfering factors such as blood in the sample matrix. In the present investigation, a specific aptamer binding was chosen for its affinity, while exhibiting no binding affinity towards non-target bacterial cells. This selective binding property was leveraged to facilitate the production of magnetic microparticles decorated with aptamers. A novel assay was developed to effectively isolate S. pneumoniae from PBS or directly from blood samples using an aptamer with an affinity constant of 72.8 nM. The capture experiments demonstrated efficiencies up to 87% and 66% are achievable for isolating spiked S. pneumoniae in 1 mL PBS and blood samples, respectively.
生物传感器发展中的一个重要限制因素是信噪比，样品基质中的干扰因素（如血液）会对其产生不利影响。在本研究中，选择了一种具有特异性的适配体结合物，因其具有亲和力，同时对非目标细菌细胞无结合亲和力。利用这种选择性结合特性，制备了用适配体修饰的磁性微粒。开发了一种新的检测方法，使用亲和力常数为72.8 nM的适配体，可从磷酸盐缓冲盐水（PBS）或直接从血液样本中有效分离肺炎链球菌。捕获实验表明，在1 mL PBS和血液样本中分离加标的肺炎链球菌时，效率分别可达87%和66%。
