Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via G. Amendola 165/A, 70126 Bari, Italy.
Genes (Basel). 2024 Jan 27;15(2):168. doi: 10.3390/genes15020168.
In the quest for sustainable and nutritious food sources, exploration of ancient grains and wild relatives of cultivated cereals has gained attention. , a wild wheatgrass species, stands out as a promising genetic resource due to its potential for crop enhancement and intriguing nutritional properties. This manuscript investigates the gene sequence and protein structure of , employing comparative analysis with other grass species to identify potential differences impacting β-glucan content. The study involves comprehensive isolation and characterization of the gene in , utilizing genomic sequence analysis, protein structure prediction, and comparative genomics. Comparisons with sequences from diverse monocots reveal evolutionary relationships, highlighting high identities with wheat genomes. Specific amino acid motifs in the CslF6 enzyme sequence, particularly those proximal to key catalytic motifs, exhibit variations among monocot species. These differences likely contribute to alterations in β-glucan composition, notably impacting the DP3:DP4 ratio, which is crucial for understanding and modulating the final β-glucan content. The study positions uniquely within the evolutionary landscape of among monocots, suggesting potential genetic divergence or unique functional adaptations within this species. Overall, this investigation enriches our understanding of β-glucan biosynthesis, shedding light on the role of specific amino acid residues in modulating enzymatic activity and polysaccharide composition.
在探索可持续和营养丰富的食物来源的过程中，人们对古老谷物和栽培谷物的野生亲属进行了研究。偃麦草属是一种野生小麦草，由于其对作物改良的潜力和有趣的营养特性，成为一种很有前途的遗传资源。本文研究了偃麦草属的 基因序列和蛋白质结构，通过与其他禾本科植物进行比较分析，确定了可能影响β-葡聚糖含量的潜在差异。该研究包括对偃麦草属中的 基因进行全面的分离和鉴定，利用基因组序列分析、蛋白质结构预测和比较基因组学。与来自不同单子叶植物的序列进行比较，揭示了进化关系，突出了与小麦基因组的高度同一性。CslF6 酶序列中的特定氨基酸模体，特别是靠近关键催化模体的那些，在单子叶植物物种中表现出变异。这些差异可能导致β-葡聚糖组成的改变，特别是对 DP3:DP4 比值的影响，这对于理解和调节最终的β-葡聚糖含量至关重要。该研究将 置于单子叶植物中 CslF6 基因的进化景观中，表明该物种内可能存在遗传分化或独特的功能适应性。总的来说，这项研究丰富了我们对β-葡聚糖生物合成的理解，揭示了特定氨基酸残基在调节酶活性和多糖组成中的作用。
