Department of Biological Sciences, University of Lethbridge, Lethbridge, AB T1K 3M4, Canada.
Genes (Basel). 2024 Jan 29;15(2):183. doi: 10.3390/genes15020183.
Serotonin emerges as a pivotal factor influencing the growth and functionality of β-cells. Psilocybin, a natural compound derived from mushrooms of the genus, exerts agonistic effects on the serotonin 5-HT2A and 5-HT2B receptors, thereby mimicking serotonin's behavior. This study investigates the potential impacts of psilocybin on β-cell viability, dedifferentiation, and function using an in vitro system. The INS-1 832/13 Rat Insulinoma cell line underwent psilocybin pretreatment, followed by exposure to high glucose-high lipid (HG-HL) conditions for specific time periods. After being harvested from treated cells, total transcript and cellular protein were utilized for further investigation. Our findings implied that psilocybin administration effectively mitigates HG-HL-stimulated β-cell loss, potentially mediated through the modulation of apoptotic biomarkers, which is possibly related to the mitigation of TXNIP, STAT-1, and STAT-3 phosphorylation. Furthermore, psilocybin exhibits the capacity to modulate the expression of key genes associated with β-cell dedifferentiation, including and , indicating its potential in attenuating β-cell dedifferentiation. This research lays the groundwork for further exploration into the therapeutic potential of psilocybin in Type II diabetes intervention.
血清素作为影响β细胞生长和功能的关键因素而出现。裸盖菇素是一种从蘑菇属中提取的天然化合物，对血清素 5-HT2A 和 5-HT2B 受体具有激动作用，从而模拟血清素的行为。本研究使用体外系统研究了裸盖菇素对β细胞活力、去分化和功能的潜在影响。INS-1 832/13 大鼠胰岛素瘤细胞系进行了裸盖菇素预处理，然后暴露于高糖高脂 (HG-HL) 条件下特定时间。从处理过的细胞中收获后，使用总转录物和细胞蛋白进行进一步研究。我们的研究结果表明，裸盖菇素的给药可有效减轻 HG-HL 刺激的β细胞丢失，这可能是通过调节凋亡生物标志物介导的，这可能与 TXNIP、STAT-1 和 STAT-3 磷酸化的减轻有关。此外，裸盖菇素还具有调节与β细胞去分化相关的关键基因表达的能力，包括 和 ，表明其在减轻β细胞去分化方面具有潜力。这项研究为进一步探索裸盖菇素在 II 型糖尿病干预中的治疗潜力奠定了基础。
