Cellular Oncology Group, Biogipuzkoa Health Research Institute, 20014 San Sebastián, Spain.
Freshage Research Group, Faculty of Medicine, Fundación Investigación Hospital Clínico Universitario/Health Research Institute INCLIVA, University of Valencia, 46010 Valencia, Spain.
Biomolecules. 2024 Jan 31;14(2):166. doi: 10.3390/biom14020166.
Frailty is a geriatric syndrome associated with negative health outcomes that represents a dynamic condition with a potential of reversibility after physical exercise interventions. Typically, inflammatory and senescence markers are increased in frail individuals. However, the impact that physical exercise exerts on inflammatory and senescence biomarkers remains unknown. We assessed the effect of physical intervention in old individuals and mice and determined the expression of inflammatory and senescence markers.
Twelve elderly individuals were enrolled from a primary care setting to a 3-month intervention. Frailty was measured by SPPB and the expression of biomarkers by cytokine array and RT-qPCR. In addition, 12 aged mice completed an intervention, and inflammation and senescence markers were studied.
The physical intervention improved the SPPB score, reducing frail and pre-frail individuals. This was correlated with a reduction in several pro-inflammatory biomarkers such as IL-6, CXCL-1, CXCL-10, IL-1β, IL-7, GM-CSF as well as and senescence markers. Otherwise, the levels of anti-inflammatory biomarker IL-4 were significantly increased. Moreover, the physical intervention in mice also improved their functional capacity and restored the expression of inflammatory (, , , and ) and senescence () markers. Additionally, PLSDA and ROC curve analysis revealed CXCL-10 and IL-1β to be the biomarkers of functional improvement in both cohorts.
Our results showed that a physical intervention improves physical frailty, and reverses inflammation and senescence biomarkers comprising CXCL-10 and IL-1β.
衰弱是一种与负面健康结果相关的老年综合征，代表一种动态状态，在进行身体锻炼干预后具有逆转的可能性。通常，衰弱个体的炎症和衰老标志物会增加。然而，身体锻炼对炎症和衰老生物标志物的影响尚不清楚。我们评估了身体干预对老年人和小鼠的影响，并确定了炎症和衰老标志物的表达。
从初级保健环境中招募了 12 名老年人参加为期 3 个月的干预。通过 SPPB 测量衰弱，通过细胞因子阵列和 RT-qPCR 测量生物标志物的表达。此外，12 只老年小鼠完成了干预，并研究了炎症和衰老标志物。
身体干预提高了 SPPB 评分，减少了虚弱和衰弱前期的个体。这与几种促炎生物标志物（如 IL-6、CXCL-1、CXCL-10、IL-1β、IL-7、GM-CSF）的减少以及衰老标志物有关。另一方面，抗炎生物标志物 IL-4 的水平显著增加。此外，小鼠的身体干预也提高了它们的功能能力，并恢复了炎症（、、、和）和衰老（）标志物的表达。此外，PLSDA 和 ROC 曲线分析显示，CXCL-10 和 IL-1β 是两个队列中功能改善的生物标志物。
我们的结果表明，身体干预可改善身体虚弱，并逆转炎症和衰老生物标志物，包括 CXCL-10 和 IL-1β。
