Wion D, Barrand P, Dicou E, Scott J, Brachet P
FEBS Lett. 1985 Sep 9;189(1):37-41. doi: 10.1016/0014-5793(85)80837-1.
Mouse L cells synthesize and secrete a neurotrophic factor related to the beta subunit of the submaxillary gland nerve growth factor (NGF) of male mice. Use of a cDNA probe which encodes the beta-NGF mRNA demonstrated that L cells produce a transcript identical in size to that of the submaxillary gland. Moreover, target sites of restriction enzymes EcoRI, PstI and BamHI were not significantly rearranged in the beta-NGF gene locus of these cells. The abundance of the beta-NGF transcript was found to depend on culture conditions. Removal of serum depressed the cellular content of polyadenylated RNA by a factor of 1.7, and decreased specifically the pool of beta-NGF transcript by an additional factor of 4. The presence of 10(-7) M testosterone in the serum-free medium did not modify the level of beta-NGF mRNA, while addition of 10(-7) M T3 (or T4) increased this level by a factor of 1.5. These data provide the first evidence that the beta-NGF mRNA of L cells is subjected to regulation, but in a way apparently different from that described for the submaxillary gland.
小鼠L细胞合成并分泌一种与雄性小鼠颌下腺神经生长因子（NGF）的β亚基相关的神经营养因子。使用编码β-NGF mRNA的cDNA探针表明，L细胞产生的转录本大小与颌下腺的相同。此外，这些细胞的β-NGF基因座中限制性内切酶EcoRI、PstI和BamHI的靶位点没有明显重排。发现β-NGF转录本的丰度取决于培养条件。去除血清使聚腺苷酸化RNA的细胞含量降低了1.7倍，并使β-NGF转录本的库进一步降低了4倍。无血清培养基中存在10^(-7) M睾酮不会改变β-NGF mRNA的水平，而添加10^(-7) M T3（或T4）会使该水平提高1.5倍。这些数据首次证明L细胞的β-NGF mRNA受到调控，但其方式明显不同于颌下腺中描述的方式。
