用于研究锌基可降解金属生物材料的hFOB 1.19细胞系的表征

Characterization of hFOB 1.19 Cell Line for Studying Zn-Based Degradable Metallic Biomaterials.

作者信息

Jablonská Eva, Mrázková Lucie, Kubásek Jiří, Vojtěch Dalibor, Paulin Irena, Ruml Tomáš, Lipov Jan

机构信息

Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.

Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.

出版信息

Materials (Basel). 2024 Feb 16;17(4):915. doi: 10.3390/ma17040915.

DOI:10.3390/ma17040915

PMID:38399166

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10890055/

Abstract

testing is the first important step in the development of new biomaterials. The human fetal osteoblast cell line hFOB 1.19 is a very promising cell model; however, there are vast discrepancies in cultivation protocols, especially in the cultivation temperature and the presence of the selection reagent, geneticin (G418). We intended to use hFOB 1.19 for the testing of Zn-based degradable metallic materials. However, the sensitivity of hFOB 1.19 to zinc ions has not yet been studied. Therefore, we compared the toxicity of zinc towards hFOB 1.19 under different conditions and compared it with that of the L929 mouse fibroblast cell line. We also tested the cytotoxicity of three types of Zn-based biomaterials in two types of media. The presence of G418 used as a selection reagent decreased the sensitivity of hFOB 1.19 to Zn. hFOB 1.19 cell line was more sensitive to Zn at elevated (restrictive) temperatures. hFOB 1.19 cell line was less sensitive to Zn than L929 cell line (both as ZnCl and extracts of alloys). Therefore, the appropriate cultivation conditions of hFOB 1.19 during biomaterial testing should be chosen with caution.

摘要

测试是新型生物材料研发过程中的首要重要步骤。人胎儿成骨细胞系hFOB 1.19是一种非常有前景的细胞模型；然而，培养方案存在巨大差异，尤其是在培养温度和选择试剂遗传霉素（G418）的使用方面。我们打算使用hFOB 1.19来测试锌基可降解金属材料。然而，hFOB 1.19对锌离子的敏感性尚未得到研究。因此，我们比较了不同条件下锌对hFOB 1.19的毒性，并将其与L929小鼠成纤维细胞系的毒性进行了比较。我们还在两种培养基中测试了三种类型锌基生物材料的细胞毒性。用作选择试剂的G418的存在降低了hFOB 1.19对锌的敏感性。hFOB 1.19细胞系在较高（限制）温度下对锌更敏感。hFOB 1.19细胞系对锌的敏感性低于L929细胞系（无论是作为氯化锌还是合金提取物）。因此，在生物材料测试过程中，应谨慎选择hFOB 1.19的合适培养条件。