Laskus-Zakrzewska Aleksandra, Kazimierczak Paulina, Kolmas Joanna
Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland.
Independent Unit of Tissue Engineering and Regenerative Medicine, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland.
Materials (Basel). 2021 Sep 4;14(17):5068. doi: 10.3390/ma14175068.
In this work, 3D porous granules based on Zn and Se-containing calcium phosphates (CaPs) were fabricated using a droplet-extrusion technique. The composite beads varied in composition and contained two different natural polymers: sodium alginate (SA) and gelatin (GEL). To analyse and compare their physicochemical properties, such as porosity and morphology, different techniques were applied, including scanning electron microscopy (SEM), sorption of N and mercury porosimetry. Prior to the fabrication of the granules, the properties of CaPs materials, (the bioceramic base of the beads), selenium (IV)-substituted hydroxyapatite (Se-HA) and zinc-substituted dicalcium phosphate dihydrate (Zn-DCPD), were also investigated. The results of cell viability assessment showed that Se-HA powder was non-toxic to human osteoblasts (hFOB 1.19) and simultaneously exhibited high toxicity to tumour cells (Saos-2). Once the cytotoxicity assay was completed, Se-HA and Zn-DCPD were used to prepare 3D materials. The prepared porous granules were used as matrices to deliver simvastatin to bones. Simvastatin was applied in either the lipophilic form or hydrophilic form. The release kinetics of simvastatin from granules of different composition was then assessed and compared.
在这项工作中，采用液滴挤出技术制备了基于含锌和硒的磷酸钙（CaP）的三维多孔颗粒。复合珠粒的成分各不相同，包含两种不同的天然聚合物：海藻酸钠（SA）和明胶（GEL）。为了分析和比较它们的物理化学性质，如孔隙率和形态，应用了不同的技术，包括扫描电子显微镜（SEM）、氮吸附和压汞法。在制备颗粒之前，还研究了CaP材料（珠粒的生物陶瓷基质）、硒（IV）取代的羟基磷灰石（Se-HA）和锌取代的二水磷酸二钙（Zn-DCPD）的性能。细胞活力评估结果表明，Se-HA粉末对人成骨细胞（hFOB 1.19）无毒，同时对肿瘤细胞（Saos-2）表现出高毒性。细胞毒性试验完成后，使用Se-HA和Zn-DCPD制备三维材料。制备的多孔颗粒用作将辛伐他汀递送至骨骼的基质。辛伐他汀以亲脂性形式或亲水性形式应用。然后评估并比较了不同组成颗粒中辛伐他汀的释放动力学。
