Department of Biosciences, University of Salzburg, Hellbrunnerstr. 34, 5020, Salzburg, Austria.
Cedars-Sinai Medical Center, Dept. of Medicine, 8700 Beverly Blvd b106, Los Angeles, CA, 90048, USA.
Sci Rep. 2021 May 25;11(1):10933. doi: 10.1038/s41598-021-90161-2.
The human fetal osteoblast cell line (hFOB 1.19) has been proposed as an accessible experimental model for study of osteoblast biology relating to drug development and biomaterial engineering. For their multilineage differentiation potential, hFOB has been compared to human mesenchymal progenitor cells and used to investigate bone-metabolism in vitro. Hereby, we studied whether and to what extent the conditionally immortalized cell line hFOB 1.19 can serve as a surrogate model for bone-marrow derived mesenchymal stromal cells (bmMSC). hFOB indeed exhibit specific characteristics reminiscent of bmMSC, as colony formation, migration capacity and the propensity to grow as multicellular aggregates. After prolonged culture, in contrast to the expected effect of immortalization, hFOB acquired a delayed growth rate. In close resemblance to bmMSC at increasing passages, also hFOB showed morphological abnormalities, enlargement and finally reduced proliferation rates together with enhanced expression of the cell cycle inhibitors p21 and p16. hFOB not only have the ability to undergo multilineage differentiation but portray several important aspects of human bone marrow mesenchymal stromal cells. Superior to primary MSC and osteoblasts, hFOB enabled the generation of continuous cell lines. These provide an advanced basis for investigating age-related dysfunctions of MSCs in an in vitro 3D-stem cell microenvironment.
人胚成骨细胞系（hFOB 1.19）已被提议作为一种可用于研究与药物开发和生物材料工程相关的成骨生物学的实验模型。由于其多能分化潜力，hFOB 已被与人骨髓间充质祖细胞进行比较，并用于体外研究骨代谢。在此，我们研究了条件永生化细胞系 hFOB 1.19 是否以及在何种程度上可以作为骨髓间充质基质细胞（bmMSC）的替代模型。hFOB 确实表现出与 bmMSC 相似的特定特征，如集落形成、迁移能力和作为多细胞聚集物生长的倾向。与永生化的预期效果相反，经过长时间培养后，hFOB 的生长速度会延迟。与 bmMSC 相似，随着传代次数的增加，hFOB 也表现出形态异常、增大，最终增殖率降低，同时细胞周期抑制剂 p21 和 p16 的表达增强。hFOB 不仅具有多能分化的能力，还具有人类骨髓间充质基质细胞的几个重要特征。与原代 MSC 和成骨细胞相比，hFOB 能够生成连续的细胞系。这些为在体外 3D 干细胞微环境中研究 MSC 与年龄相关的功能障碍提供了先进的基础。
