Shapiro R E, Miselis R R
J Comp Neurol. 1985 Aug 22;238(4):473-88. doi: 10.1002/cne.902380411.
We employed the neural tracers cholera toxin-horseradish peroxidase and wheat germ agglutinin-horseradish peroxidase to examine the organization of the afferent and efferent connections of the stomach within the medulla oblongata of the rat. The major finding of this study is that gastric motoneurons of the dorsal motor nucleus (DMN) possess numerous dendrites penetrating discrete regions of the overlying nucleus of the solitary tract (NTS). In particular, dendritic labelling was present in areas of NTS which also received terminals of gastric vagal afferent fibers such as the subnucleus gelatinosus, nucleus commissuralis, and medial nucleus of NTS. This codistribution of afferent and efferent elements of the gastric vagus may provide loci for monosynaptic vagovagal interactions. A small number of dendrites of DMN neurons penetrated the ependyma of the fourth ventricle and a few others entered the ventral aspect of the area postrema, thus making possible the direct contact of preganglionic neurons with humoral input from the cerebrospinal fluid and/or the peripheral plasma. Nucleus ambiguus neurons projecting to the stomach predominantly innervate the forestomach. The dendrites of these cells, when labelled, were generally short, and extended beyond the compact cluster of ambiguus neurons in a ventrolateral direction, parallel to the fascicles of vagal efferent fibers traversing the medulla.
我们使用神经示踪剂霍乱毒素-辣根过氧化物酶和小麦胚凝集素-辣根过氧化物酶，来研究大鼠延髓内胃的传入和传出连接的组织情况。本研究的主要发现是，背运动核（DMN）的胃运动神经元拥有众多树突，这些树突穿透了上方孤束核（NTS）的离散区域。特别是，在NTS中也接受胃迷走神经传入纤维终末的区域，如胶状质、连合核和NTS内侧核，存在树突标记。胃迷走神经传入和传出成分的这种共分布可能为单突触迷走神经相互作用提供位点。DMN神经元的少数树突穿透第四脑室的室管膜，还有一些进入最后区的腹侧，从而使节前神经元能够直接接触来自脑脊液和/或外周血浆的体液输入。投射到胃的疑核神经元主要支配前胃。这些细胞的树突在被标记时通常较短，并在腹外侧方向延伸到疑核神经元紧密簇之外，与穿过延髓的迷走神经传出纤维束平行。
