Chen Y T, Obata Y, Stockert E, Old L J
J Exp Med. 1985 Oct 1;162(4):1134-48. doi: 10.1084/jem.162.4.1134.
A thymus-leukemia (TL)-specific probe, pTL1, has been generated from a TL-coding gene of BALB/c mice. Multiple species of TL mRNA were detected in TL+ cells by Northern blot analysis with pTL1, and different Tla haplotypes could be distinguished on the basis of characteristic patterns of TL mRNA. No TL-related message was found in normal or leukemic TL- cells, including thymocytes from Tlab mice. However, TL mRNA could be detected in TL+ leukemias occurring in Tlab mice. A cDNA library has been made from ASL1 (a TL+ leukemia of A mice [Tlaa]), and pTL1+ clones have been sequenced. At least three structurally distinct TL genes are expressed in ASL1. Sequence comparison of TL genes from three Tla haplotypes indicates that TL genes are highly conserved (greater than 90% homology) and are more distantly related to H-2 genes. Several polyadenylation sites have been found in the 3' untranslated region of TL genes, and differential polyadenylation contributes to the size heterogeneity of TL transcripts. The predicted amino acid sequence of TL products indicates that TL and H-2 are similar in domain structure and disulfide bonds, but differ in glycosylation sites and in cytoplasmic domain sequences.
一种胸腺白血病（TL）特异性探针pTL1，是从BALB/c小鼠的TL编码基因中产生的。用pTL1进行Northern印迹分析，在TL+细胞中检测到多种TL mRNA，并且可以根据TL mRNA的特征模式区分不同的Tla单倍型。在正常或白血病性TL-细胞中未发现与TL相关的信息，包括来自Tlab小鼠的胸腺细胞。然而，在Tlab小鼠中发生的TL+白血病中可以检测到TL mRNA。已经从ASL1（A小鼠的TL+白血病[Tlaa]）构建了一个cDNA文库，并对pTL1+克隆进行了测序。在ASL1中至少表达了三种结构不同的TL基因。来自三种Tla单倍型的TL基因的序列比较表明，TL基因高度保守（同源性大于90%），并且与H-2基因的关系更远。在TL基因的3'非翻译区发现了几个聚腺苷酸化位点，差异聚腺苷酸化导致了TL转录本的大小异质性。TL产物的预测氨基酸序列表明，TL和H-2在结构域结构和二硫键方面相似，但在糖基化位点和细胞质结构域序列方面不同。
