Carlin G, Djursäter R, Smedegård G, Gerdin B
Agents Actions. 1985 Jul;16(5):377-84. doi: 10.1007/BF01982876.
The inhibitory effect of various anti-inflammatory drugs on the xanthine oxidase derived depolymerization of hyaluronic acid was studied. The depolymerization was assayed by repeated viscosity measurements. By using a low xanthine oxidase activity, the decrease in viscosity with time followed first order reaction kinetics and was therefore suitable for kinetic analysis. The xanthine oxidase activity was monitored by assay of O2-consumption with a Clark-electrode and by assay of urate production. We present evidence that salicylic, acetylsalicylic, gentisic and azodisalicylic acid and sulfasalazine inhibit the production of oxygen-derived free radicals by xanthine oxidase. We found that sulfapyridine, 5-aminosalicylic acid, allopurinol, mannitol, glucuronic acid and N-acetylglucosamine in addition to the earlier studied drugs, paracetamol, ibuprofen, benoxaprofen and gentisic acid exert their effect via scavenging of free radicals. These drugs had very little effect on the enzyme activity.
研究了各种抗炎药物对黄嘌呤氧化酶介导的透明质酸解聚的抑制作用。通过反复测量粘度来测定解聚情况。使用低活性的黄嘌呤氧化酶时，粘度随时间的降低符合一级反应动力学，因此适用于动力学分析。通过用克拉克电极测定氧气消耗以及测定尿酸生成来监测黄嘌呤氧化酶的活性。我们提供的证据表明，水杨酸、乙酰水杨酸、龙胆酸、偶氮二水杨酸和柳氮磺胺吡啶可抑制黄嘌呤氧化酶产生氧自由基。我们发现，除了先前研究过的药物对乙酰氨基酚、布洛芬、苯恶洛芬和龙胆酸外，磺胺吡啶、5-氨基水杨酸、别嘌呤醇、甘露醇、葡萄糖醛酸和N-乙酰葡糖胺通过清除自由基发挥作用。这些药物对酶活性的影响很小。
