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全基因组鉴定 N6-甲基腺苷调控基因揭示 NtFIP37B 增强烟草(Nicotiana tabacum L.)的抗旱性。

Genome-wide identification of the N-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.).

机构信息

Beijing Life Science Academy, Beijing, 102200, China.

China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, 450001, China.

出版信息

BMC Plant Biol. 2024 Feb 26;24(1):134. doi: 10.1186/s12870-024-04813-2.

Abstract

BACKGROUND

N-methyladenosine (mA) is one of the common internal RNA modifications found in eukaryotes. The mA modification can regulate various biological processes in organisms through the modulation of alternative splicing, alternative polyadenylation, folding, translation, localization, transport, and decay of multiple types of RNA, without altering the nucleotide sequence. The three components involved in mA modification, namely writer, eraser, and reader, mediate the abundance of RNA mA modification through complex collaborative actions. Currently, research on mA regulatory genes in plants is still in its infancy.

RESULTS

In this study, we identified 52 candidate mA regulatory genes in common tobacco (Nicotiana tabacum L.). Gene structure, conserved domains, and motif analysis showed structural and functional diversity among different subgroups of tobacco mA regulatory genes. The amplification of mA regulatory genes were mainly driven by polyploidization and dispersed duplication, and duplicated genes evolved through purified selection. Based on the potential regulatory network and expression pattern analysis of mA regulatory genes, a significant number of mA regulatory genes might play important roles in growth, development, and stress response processes. Furthermore, we have confirmed the critical role of NtFIP37B, an mA writer gene in tobacco, in enhancing drought resistance.

CONCLUSIONS

This study provides useful information for better understanding the evolution of mA regulatory genes and the role of mA modification in tobacco stress response, and lays the foundation for further elucidating the function of mA regulatory genes in tobacco.

摘要

背景

N6-甲基腺苷(mA)是真核生物中常见的内部 RNA 修饰之一。mA 修饰可以通过调节多种类型 RNA 的可变剪接、可变多聚腺苷酸化、折叠、翻译、定位、运输和降解,来调控生物体内的各种生理过程,而不改变核苷酸序列。mA 修饰的三个参与成分,即 writer、eraser 和 reader,通过复杂的协同作用来调节 RNA mA 修饰的丰度。目前,植物中 mA 调控基因的研究仍处于起步阶段。

结果

本研究在普通烟草(Nicotiana tabacum L.)中鉴定出 52 个候选 mA 调控基因。基因结构、保守结构域和基序分析显示,不同亚组烟草 mA 调控基因具有结构和功能的多样性。mA 调控基因的扩增主要由多倍化和分散重复驱动,重复基因通过纯化选择进化。基于 mA 调控基因的潜在调控网络和表达模式分析,大量 mA 调控基因可能在生长、发育和应激响应过程中发挥重要作用。此外,我们已经证实了烟草 mA 写作者基因 NtFIP37B 在增强耐旱性方面的关键作用。

结论

本研究为更好地理解 mA 调控基因的进化以及 mA 修饰在烟草应激响应中的作用提供了有用的信息,并为进一步阐明烟草 mA 调控基因的功能奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6934/10895791/a74eb22d90f2/12870_2024_4813_Fig1_HTML.jpg

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