Wu Wenyan, Wang Xinhua, Liang Xingrui, Huang Xinqi, Nawaz Muhammad Amjad, Jing Chenchen, Fan Yaru, Niu Jingya, Wu Jing, Feng Xue
College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China.
Advanced Engineering School (Agrobiotek), National Research Tomsk State University, Lenin Ave, 36, 634050 Tomsk, Tomsk Oblast, Russia.
Int J Mol Sci. 2025 Mar 19;26(6):2748. doi: 10.3390/ijms26062748.
Common bean ( L.) is known for its high protein, dietary fiber, and various trace element contents, making it a widely grown leguminous crop globally. The bean common mosaic virus (BCMV) poses a significant threat to leguminous crop production, causing substantial yield reductions when common beans are infected. Widely occurring in mRNA, the mA modification is vital for maintaining mRNA stability, facilitating splicing, enabling nuclear export, supporting polyadenylation, and initiating translation. Recent studies have identified the mA regulatory gene family in various plant species, and its ability to regulate plant virus infection has been confirmed. There is currently insufficient information regarding the mA regulatory gene family in beans and how it responds to BCMV infection. Consequently, we carried out a genome-wide characterization of the mA regulatory gene family in common bean, which led to the identification of 31 potential regulatory gene members associated with mA. According to evolutionary analysis, the increase in the bean mA regulatory gene family appears to be linked to either whole-genome duplication or segmental duplication events. Subsequent investigations into the expression levels of these genes throughout different phases of BCMV infection showed that all candidate genes responded to the infection with various changes in expression. Moreover, we characterized the methyltransferase activity of PvMTA and validated the interactive relationship between mRNA adenosine methyltransferase A (MTA) and mRNA adenosine methyltransferase B (MTB) in common beans. Through overexpressing and silencing , we further ascertained that this particular gene has a detrimental impact on the regulation of BCMV infection. This research provides fresh perspectives on the molecular processes that govern the interaction between the common bean and BCMV and aids progress in molecular bean breeding.
菜豆（Phaseolus vulgaris L.）以其高蛋白、膳食纤维和多种微量元素含量而闻名，是全球广泛种植的豆科作物。菜豆普通花叶病毒（BCMV）对豆科作物生产构成重大威胁，菜豆感染后会导致大幅减产。mA修饰广泛存在于mRNA中，对维持mRNA稳定性、促进剪接、实现核输出、支持多聚腺苷酸化和启动翻译至关重要。最近的研究已在多种植物物种中鉴定出mA调控基因家族，并证实了其调控植物病毒感染的能力。目前关于菜豆中mA调控基因家族及其对BCMV感染的反应的信息不足。因此，我们对菜豆中的mA调控基因家族进行了全基因组特征分析，鉴定出31个与mA相关的潜在调控基因成员。根据进化分析，菜豆mA调控基因家族的增加似乎与全基因组复制或片段重复事件有关。随后对这些基因在BCMV感染不同阶段的表达水平进行的研究表明，所有候选基因都对感染有不同的表达变化反应。此外，我们对PvMTA的甲基转移酶活性进行了表征，并验证了菜豆中mRNA腺苷甲基转移酶A（MTA）和mRNA腺苷甲基转移酶B（MTB）之间的相互作用关系。通过过表达和沉默，我们进一步确定该特定基因对BCMV感染的调控有不利影响。这项研究为控制菜豆与BCMV相互作用的分子过程提供了新的视角，并有助于菜豆分子育种的进展。
