Cheng Lingtong, Ma Lanxin, Meng Lijun, Shang Haihong, Cao Peijian, Jin Jingjing
China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.
Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China.
Front Genet. 2022 Jun 13;13:916867. doi: 10.3389/fgene.2022.916867. eCollection 2022.
Class III peroxidases (PODs) are plant-specific enzymes that play significant roles in plant physiological processes and stress responses. However, a comprehensive analysis of the POD gene family in tobacco has not yet been conducted. In this study, 210 non-redundant POD gene members () were identified in tobacco () and distributed unevenly throughout 24 tobacco chromosomes. Phylogenetic analysis clustered these genes into six subgroups (I-VI). Gene structure and motif analyses showed the structural and functional diversity among the subgroups. Segmental duplication and purifying selection were the main factors affecting gene evolution. Our analyses also suggested that s might be regulated by miRNAs and cis-acting regulatory elements of transcription factors that are involved in various biological processes. In addition, the expression patterns in different tissues and under various stress treatments were investigated. The results showed that the majority of had tissue-specific expression patterns and may be involved in many biotic and abiotic responses. qRT-PCR analyses of different tissues and stress treatments were performed to verify transcriptome patterns. Expression of a green fluorescent protein-NtPOD fusion confirmed the plasma membrane localization of NtPOD121 and NtPOD4. Furthermore, 3D structures provided evidences of membrane-bound peroxidase. These findings provide useful information to better understand the evolution of the gene family and lay the foundation for further studies on POD gene function in tobacco.
Ⅲ类过氧化物酶（PODs）是植物特有的酶，在植物生理过程和应激反应中发挥着重要作用。然而，尚未对烟草中的POD基因家族进行全面分析。在本研究中，在烟草中鉴定出210个非冗余POD基因成员，并在24条烟草染色体上分布不均。系统发育分析将这些基因聚类为六个亚组（I-VI）。基因结构和基序分析显示了亚组之间的结构和功能多样性。片段重复和纯化选择是影响基因进化的主要因素。我们的分析还表明，PODs可能受参与各种生物过程的miRNA和转录因子顺式作用调控元件的调节。此外，研究了不同组织和各种胁迫处理下的表达模式。结果表明，大多数PODs具有组织特异性表达模式，可能参与许多生物和非生物反应。对不同组织和胁迫处理进行qRT-PCR分析以验证转录组模式。绿色荧光蛋白-NtPOD融合蛋白的表达证实了NtPOD121和NtPOD4的质膜定位。此外，三维结构提供了膜结合过氧化物酶的证据。这些发现为更好地理解POD基因家族的进化提供了有用信息，并为进一步研究烟草中POD基因的功能奠定了基础。
