基于弱测量的光学生物传感器用于超灵敏检测人血清中的钙网蛋白。
Optical biosensor based on weak measurement for ultra-sensitive detection of calreticulin in human serum.
作者信息
Peng Zhikang, Chen Jiali, Liu Yufei, Li Dongmei, Li Yixiao, Zhang Qihao, Chen Chaoyi, Zhang Ying, Yao Jia, Wang Shuqian, Lin Qiang, Ruan Yi
机构信息
Key Laboratory of Quantum Precision Measurement of Zhejiang Province, Center for Optics & Optoelectronics Research, Collaborative Innovation Center for Information Technology in Biological and Medical Physics, College of Science, Zhejiang University of Technology, Hangzhou 310023, China.
Department of Endocrinology and Metabolism, Shaoxing Second Hospital, Shaoxing 312000, China.
出版信息
Biomed Opt Express. 2024 Jan 16;15(2):715-724. doi: 10.1364/BOE.514443. eCollection 2024 Feb 1.
A novel real-time optical phase sensing method based on the Mach-Zehnder interference principle has been proposed for the detection of calreticulin (CRT) levels in human serum samples. In this approach, anti-CRT antibodies are utilized to capture CRT molecules in serum, leading to a phase shift in both the measuring and reference arms of the system. By employing the concept of weak amplification within the framework of weak measurements, it becomes feasible to continuously monitor the response of CRT in real-time, allowing for the precise determination of serum CRT content at the picomolar level. Our achievement may pave the way in establishing CRT as a diagnostic biomarker for a wide range of medical applications, including rheumatoid arthritis.
一种基于马赫-曾德尔干涉原理的新型实时光学相位传感方法已被提出，用于检测人血清样本中的钙网蛋白（CRT）水平。在这种方法中，抗CRT抗体被用于捕获血清中的CRT分子，导致系统测量臂和参考臂都发生相移。通过在弱测量框架内采用弱放大概念，可以实时连续监测CRT的响应，从而能够精确测定皮摩尔水平的血清CRT含量。我们的成果可能为将CRT确立为包括类风湿关节炎在内的广泛医学应用中的诊断生物标志物铺平道路。
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