Asamizu T, Summers D, Motika M B, Anzola J V, Nuss D L
Virology. 1985 Jul 30;144(2):398-409. doi: 10.1016/0042-6822(85)90281-8.
The double-stranded RNA genome of the tumor-inducing plant pathogen, wound tumor virus, was converted to double-stranded DNA and cloned into plasmid pBR322. Multiple apparent full-length copies of 9 of the 12 wound tumor virus genome segments were identified. The entire sequence of cloned genome segment S12, the smallest of the genome segments, was determined. This genome segment was found to be 851 nucleotides in length and to possess a single long open reading frame that extends 178 codons from the first AUG triplet (residues 35-37): information sufficient to encode a protein of the size estimated for the smallest of the previously identified wound tumor virus primary gene products, Pns 12. Sequence data obtained from analysis of cloned cDNA copies of several genome segments and from direct analysis of the 3' termini of the double-stranded genome RNAs revealed that each wound tumor virus genome segment possesses the common terminal sequences: (+) 5'GGUAUU ... UGAU 3' (-) 3'CCAUAA ... ACUA 5'.
致瘤植物病原体伤口瘤病毒的双链RNA基因组被转化为双链DNA，并克隆到质粒pBR322中。在12个伤口瘤病毒基因组片段中，鉴定出了9个片段的多个明显全长拷贝。测定了克隆的基因组片段S12（基因组中最小的片段）的完整序列。该基因组片段长度为851个核苷酸，具有一个单一的长开放阅读框，从第一个AUG三联体（第35 - 37位残基）延伸178个密码子：这些信息足以编码一个大小与先前鉴定的伤口瘤病毒主要基因产物中最小的Pns 12估计大小相同的蛋白质。从几个基因组片段的克隆cDNA拷贝分析以及双链基因组RNA的3'末端直接分析获得的序列数据表明，每个伤口瘤病毒基因组片段都具有共同的末端序列：(+) 5'GGUAUU... UGAU 3' (-) 3'CCAUAA... ACUA 5'
