Department of Clinical Laboratory Sciences, Faculty of Pharmacy, Al-Rafidain University College, Baghdad, Iraq.
Department of Clinical Laboratory Sciences, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
J Med Life. 2023 Nov;16(11):1663-1669. doi: 10.25122/jml-2023-0181.
In individuals with type 2 diabetes mellitus (T2DM), the cannabinoid receptor 1 () gene polymorphism has been linked to diabetic nephropathy (DN). Different renal disorders, including DN, have been found to alter cannabinoid (CB) receptor expression and activation. This cross-sectional study aimed to investigate the relationship between rs1776966256 and rs1243008337 genetic variants and the risk of developing DN in Iraqi patients with T2DM. The study included 100 patients with T2DM, divided into two groups: 50 with DN and 50 without DN. Genotyping of rs1776966256 and rs1243008337 polymorphisms was conducted using PCR in DN patients and control samples. The distribution of rs1776966256 and rs1243008337 genotypes and alleles between the two groups revealed statistically significant differences. The frequencies of the GG and AG genotypes of rs1776966256 were significantly different between DN patients and the control group. Additionally, compared to the A allele, the G allele of this polymorphism was linked to a higher incidence of DN (p=0.0001). Patients with the genetic polymorphism rs1243008337 had higher genotypes of CC and AC and were more likely to develop DN in the polymorphism genotype than the wild genotype. Additionally, compared to the A allele, the C allele was linked to a higher chance of developing DN (p=0.0001). Both rs1776966256 and rs1243008337 polymorphisms were correlated with the development of diabetic nephropathy.
在 2 型糖尿病（T2DM）患者中，大麻素受体 1（）基因多态性与糖尿病肾病（DN）有关。已经发现不同的肾脏疾病，包括 DN，会改变大麻素（CB）受体的表达和激活。这项横断面研究旨在探讨 rs1776966256 和 rs1243008337 基因变异与伊拉克 T2DM 患者发生 DN 的风险之间的关系。该研究纳入了 100 名 T2DM 患者，分为两组：50 名患有 DN，50 名没有 DN。使用 PCR 在 DN 患者和对照样本中对 rs1776966256 和 rs1243008337 多态性进行基因分型。两组间 rs1776966256 和 rs1243008337 基因型和等位基因的分布存在统计学显著差异。rs1776966256 的 GG 和 AG 基因型在 DN 患者和对照组之间的分布存在显著差异。此外，与 A 等位基因相比，该多态性的 G 等位基因与 DN 发生率较高相关（p=0.0001）。携带 rs1243008337 遗传多态性的患者具有更高的 CC 和 AC 基因型，并且在多态性基因型中比野生基因型更有可能发生 DN。此外，与 A 等位基因相比，C 等位基因与发生 DN 的几率较高相关（p=0.0001）。rs1776966256 和 rs1243008337 多态性均与糖尿病肾病的发生相关。
