Zil'fian V N, Neresian A K, Kumkumadzhian V A, Fichidzhian B S
Genetika. 1985 Sep;21(9):1507-11.
The influence of immunization with tularemic live dry vaccine applied singularly to Wistar rats skin on mutagenicity induced by cyclophosphamide, methylnitrosourea, benzo (a) pyrene, chloroprene and tetrachlorbutane was studied. The chemical compounds indicated were injected intraperitoneally at doses equal to one fifth of LD50 in 15 days after vaccination. The same doses of these chemicals were applied to intact rats (controls). Metaphase plates were prepared from rat bone marrows by known methods, 9600 metaphase cells were studied in total. The analysis showed that cytogenetic disturbances in immunized animals were significantly decreased (from 1.5 to 2.6 times), as compared to nonimmunized animals. The possible mechanisms of reducing the quantity of cytogenetic disorders in myelokaryocytes of immunized rats are discussed in this paper. The results obtained may be both of scientific and practical significance.
研究了将土拉菌活干疫苗单独接种于Wistar大鼠皮肤后，对环磷酰胺、甲基亚硝基脲、苯并(a)芘、氯丁二烯和四氯化丁烷诱导的致突变性的影响。在接种疫苗15天后，以等于LD50五分之一的剂量腹腔注射上述化学化合物。将相同剂量的这些化学物质应用于未处理的大鼠（对照组）。采用已知方法从大鼠骨髓制备中期分裂相板，共研究9600个中期细胞。分析表明，与未免疫动物相比，免疫动物的细胞遗传学紊乱显著减少（减少1.5至2.6倍）。本文讨论了免疫大鼠骨髓细胞中细胞遗传学紊乱数量减少的可能机制。所得结果可能具有科学和实际意义。
