Department of Gynecology, Jinan Maternity and Child Care Hospital, 250001 Jinan, Shandong, China.
Department of Pathology, Jinan Maternity and Child Care Hospital, 250001 Jinan, Shandong, China.
Discov Med. 2024 Feb;36(181):286-293. doi: 10.24976/Discov.Med.202436181.27.
Hydatidiform mole (HM) is a common pregnancy disease among women of gestational age. Twist-related protein 1 (Twist-1) is involved in the development of various tumors, but its role in HM is poorly defined. This study aimed to explore Twist-1 expression and its biological function in HM cells.
Twist-1 expression in HM was detected by immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR). The effects of silencing Twist-1 on choriocarcinoma (CCA) cell proliferation were detected by cell counting kit-8 (CCK-8) and clone formation assays. CCA cell migration and invasion were detected through transwell assay. Western blot was used to detect epithelial-mesenchymal transition (EMT) and the expression of phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway-related proteins.
Twist-1 expression was upregulated in HM tissues ( < 0.001) and CCA cells ( < 0.01). Twist-1 silencing inhibited proliferation of BeWo and JAR cells ( < 0.01, < 0.05) as shown by CCK-8 assay ( < 0.01) and clone formation assays ( < 0.01, < 0.05). Twist-1 silencing inhibited the migration ( < 0.01) and invasion activity ( < 0.01, < 0.05) of BeWo and JAR cells. Western blot results showed that Twist-1 silencing promoted E-cadherin ( < 0.01) expression, and inhibited N-cadherin ( < 0.01, < 0.05) and vimentin ( < 0.01, < 0.05) expression in BeWo and JAR cells. Twist-1 downregulation decreased protein levels of p-PI3K ( < 0.01) and p-AKT ( < 0.01, < 0.05) in BeWo and JAR cells.
Silencing Twist-1 inhibits the malignant behavior of CCA cells, which may play a part by inhibiting the EMT process and the PI3K/AKT pathway.
葡萄胎（HM）是一种常见的妊娠疾病，发生于育龄妇女。 twist 相关蛋白 1（Twist-1）参与多种肿瘤的发生发展，但在 HM 中的作用尚不清楚。本研究旨在探讨 Twist-1 在 HM 细胞中的表达及其生物学功能。
采用免疫组织化学和实时定量聚合酶链反应（qRT-PCR）检测 HM 中 Twist-1 的表达。通过细胞计数试剂盒-8（CCK-8）和克隆形成实验检测沉默 Twist-1 对绒毛膜癌（CCA）细胞增殖的影响。Transwell 实验检测 CCA 细胞迁移和侵袭。Western blot 检测上皮间质转化（EMT）及磷脂酰肌醇 3-激酶/蛋白激酶 B（PI3K/AKT）通路相关蛋白的表达。
HM 组织（ < 0.001）和 CCA 细胞（ < 0.01）中 Twist-1 的表达上调。沉默 Twist-1 抑制 BeWo 和 JAR 细胞的增殖（ < 0.01， < 0.05），CCK-8 实验（ < 0.01）和克隆形成实验（ < 0.01， < 0.05）。沉默 Twist-1 抑制 BeWo 和 JAR 细胞的迁移（ < 0.01）和侵袭活性（ < 0.01， < 0.05）。Western blot 结果显示，沉默 Twist-1 促进 E-钙黏蛋白（ < 0.01）的表达，抑制 BeWo 和 JAR 细胞中 N-钙黏蛋白（ < 0.01， < 0.05）和波形蛋白（ < 0.01， < 0.05）的表达。下调 Twist-1 降低了 BeWo 和 JAR 细胞中 p-PI3K（ < 0.01）和 p-AKT（ < 0.01， < 0.05）的蛋白水平。
沉默 Twist-1 抑制 CCA 细胞的恶性行为，可能通过抑制 EMT 过程和 PI3K/AKT 通路发挥作用。
