Core Lab Glycobiol & Glycoengn, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, Liaoning, China.
Department of Pathology, Dalian Municipal Women and Children's Medical Center, Dalian 116044, Liaoning, China.
Biomed Pharmacother. 2019 May;113:108760. doi: 10.1016/j.biopha.2019.108760. Epub 2019 Mar 16.
MicroRNAs (miRNAs) are a class of small non-coding RNAs that are closely associated with carcinogenesis. Accumulating data indicate that miR-196b participates in the development of various types of cancers. However, the role of miR-196b in the formation of hydatidiform mole (HM) is still unclear. Our previous studies have demonstrated that miR-196b levels were decreased in JAR and BeWo cells and in HM tissue samples, as demonstrated by RT-PCR analysis. Furthermore, we discovered that overexpression of miR-196b in JAR and BeWo cells inhibited cellular proliferation, migration and invasion, as shown by Cell counting kit-8 (CCK-8) and transwell assays, respectively. Subsequently, we explored the interaction of miR-196b with its target gene in human choriocarcinoma cell lines. MAP3K1 is a target gene predicted by bioinformatic analysis that was previously shown to exhibit reduced expression levels following treatment with miR-196b in JAR and BeWo cells. We demonstrated that MAP3K1 was a direct target of miR-196b using the dual-luciferase reporter assay in Hela cells. In summary, the present study demonstrated that miR-196b suppressed proliferation, migration and invasion of human choriocarcinoma cells by inhibiting its transcriptional target MAP3K1. miR-196b and MAP3K1 may be considered potential targets for the clinical treatment of HM.
微小 RNA（miRNAs）是一类与致癌作用密切相关的小非编码 RNA。越来越多的证据表明，miR-196b 参与了多种类型癌症的发生。然而，miR-196b 在葡萄胎（HM）形成中的作用尚不清楚。我们之前的研究表明，通过 RT-PCR 分析，miR-196b 在 JAR 和 BeWo 细胞及 HM 组织样本中的表达水平降低。此外，我们发现 JAR 和 BeWo 细胞中 miR-196b 的过表达抑制了细胞增殖、迁移和侵袭，分别通过细胞计数试剂盒-8（CCK-8）和 Transwell 分析证实。随后，我们探索了 miR-196b 与其在人绒癌细胞系中的靶基因的相互作用。MAP3K1 是生物信息学分析预测的靶基因，之前的研究表明，在 JAR 和 BeWo 细胞中用 miR-196b 处理后，其表达水平降低。我们通过在 Hela 细胞中的双荧光素酶报告基因检测证实 MAP3K1 是 miR-196b 的直接靶基因。综上所述，本研究表明，miR-196b 通过抑制其转录靶基因 MAP3K1 抑制人绒癌细胞的增殖、迁移和侵袭。miR-196b 和 MAP3K1 可能被认为是 HM 临床治疗的潜在靶点。
