Menétrey D
Histochemistry. 1985;83(5):391-5. doi: 10.1007/BF00509197.
In this study I have used a tracer complex made of wheat germ agglutinin horseradish peroxidase conjugate (WGA*HRP) coupled to colloidal gold for retrograde tracing of neuronal pathways at the light microscopic level. Visualization of the gold was achieved by silver precipitation (the gold silver intensification method) with gold particles acting as specific cores of nucleation. The presence of horseradish peroxidase in the protein conjugate allowed this method to be compared with classical histochemistry using tetramethylbenzidine as a chromogen. The gold silver intensification method proved to be reliable, specific and sensitive. It has been demonstrated to be useful with fixatives containing a high percentage of paraformaldehyde and compatible with histochemical procedures to show projections of transmitter specific pathways.
在本研究中，我使用了一种由小麦胚凝集素辣根过氧化物酶共轭物（WGA*HRP）与胶体金偶联而成的示踪复合物，用于在光学显微镜水平上对神经通路进行逆行追踪。通过银沉淀（金银强化法）实现金的可视化，金颗粒作为特定的成核核心。蛋白质共轭物中辣根过氧化物酶的存在使得该方法能够与使用四甲基联苯胺作为显色剂的经典组织化学方法进行比较。金银强化法被证明是可靠、特异且灵敏的。已证明它适用于含有高百分比多聚甲醛的固定剂，并与组织化学程序兼容，以显示递质特异性通路的投射。
