Department of Bioinformatics and Genomics, College of Computing and Informatics, the University of North Carolina at Charlotte, Charlotte, NC, 28223, USA.
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.
Sci Data. 2024 Feb 27;11(1):247. doi: 10.1038/s41597-024-03087-5.
There are four species in the Crossoptilon genus inhibiting at from very low to very high altitudes across China, and they are in varying levels of danger of extinction. To better understand the genetic basis of adaptation to high altitudes and genetic changes due to bottleneck, we assembled the genome (1.02 Gb) of a white eared pheasant (WT) (Crossoptilon crossoptilon) inhibiting at high altitudes (3,0007,000 m) in northwest of Yunnan province, China, using a combination of Illumina short reads, PacBio long reads and Hi-C reads, with a contig N50 of 19.63 Mb and only six gaps. To further provide resources for gene annotation as well as functional and population genetics analyses, we sequenced transcriptomes of 20 major tissues of the WT individual and re-sequenced another 10 WT individuals and a blue eared pheasant (Crossoptilon auritum) individual inhabiting at intermediate altitudes (1,500~3,000 m). Our assembled WT genome, transcriptome data, and DNA sequencing data can be valuable resources for studying the biology, evolution and developing conservation strategies of these endangered species.
在中国,有四个物种的 crossoptilon 属生活在从非常低到非常高的海拔地区,它们都处于不同程度的灭绝危险之中。为了更好地了解适应高海拔的遗传基础和瓶颈引起的遗传变化,我们使用 Illumina 短读长、PacBio 长读长和 Hi-C 读长组合,组装了一只生活在中国云南省西北部高海拔地区(3000-7000 米)的白耳雉(WT)(Crossoptilon crossoptilon)的基因组(约 10.2 亿碱基对),其重叠群 N50 为 19.63Mb,仅有 6 个缺口。为了进一步提供基因注释以及功能和群体遗传学分析的资源,我们对 WT 个体的 20 种主要组织进行了转录组测序,并对另外 10 个 WT 个体和一个生活在中海拔地区(1500-3000 米)的蓝耳雉(Crossoptilon auritum)个体进行了重测序。我们组装的 WT 基因组、转录组数据和 DNA 测序数据将成为研究这些濒危物种生物学、进化和制定保护策略的宝贵资源。