• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

西花蓟马气味结合蛋白的鉴定、通过分子建模、分子动力学模拟及验证性田间试验对其进行表征和结合分析。

Identification of the odorant binding proteins of Western Flower Thrips (), characterization and binding analysis of with molecular modelling, molecular dynamics simulations and a confirmatory field trial.

作者信息

Zafar Zeeshan, Wood Martyn J, Fatima Sidra, Bhatti Muhammad Faraz, Shah Farooq A, Saud Zack, Loveridge E Joel, Karaca Ismail, Butt Tariq M

机构信息

Research and Development, Razbio Limited, Bridgend, UK.

Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.

出版信息

J Biomol Struct Dyn. 2024 Feb 28:1-16. doi: 10.1080/07391102.2024.2317990.

DOI:10.1080/07391102.2024.2317990
PMID:38415377
Abstract

Olfactory systems are indispensable for insects as they, including Western Flower Thrips (), use olfactory cues for ovipositing and feeding. use odorant binding proteins (OBPs) to transport semiochemicals to odorant receptors to induce a behavioural response from the sensillum lymph of the insect's antennae. This study identifies four OBPs of and analyses their expression at three stages of growth: larvae, adult males and adult females. Further, it investigates the presence of conserved motifs and their phylogenetic relationship to other insect species. Moreover, was characterized to analyse its structure along with molecular docking and molecular dynamics simulations to understand its binding with semiochemicals of . Molecular docking revealed the interactions of methyl isonicotinate, p-anisaldehyde and (S)-(-)-verbenone with . Moreover, molecular dynamics simulations showed bonding stability of these ligands with and field trials validated that Lurem TR (commercial product) and p-anisaldehyde had greater attraction as compared to (S)-(-)-verbenone, given the compound's binding with . The current study helps in understanding the tertiary structure and interaction of with lures using computational and field data and will help in the identification of novel lures of insects in the future, given the importance of binding with OBPs.

摘要

嗅觉系统对昆虫来说是不可或缺的,因为它们(包括西花蓟马)利用嗅觉线索进行产卵和觅食。利用气味结合蛋白(OBPs)将信息化学物质运输到气味受体,以诱导昆虫触角感器淋巴产生行为反应。本研究鉴定了西花蓟马的四种OBPs,并分析了它们在幼虫、成年雄性和成年雌性三个生长阶段的表达情况。此外,还研究了保守基序的存在及其与其他昆虫物种的系统发育关系。此外,对西花蓟马的一种OBP进行了表征,分析了其结构,并结合分子对接和分子动力学模拟,以了解其与西花蓟马信息化学物质的结合情况。分子对接揭示了异烟酸甲酯、对茴香醛和(S)-(-)-马鞭草烯酮与西花蓟马的一种OBP的相互作用。此外,分子动力学模拟表明这些配体与西花蓟马的一种OBP具有结合稳定性,田间试验验证了Lurem TR(商业产品)和对茴香醛比(S)-(-)-马鞭草烯酮具有更大的吸引力,因为该化合物与西花蓟马的一种OBP有结合。目前这项研究有助于利用计算和田间数据了解西花蓟马的一种OBP的三级结构及其与诱捕剂的相互作用,鉴于与OBPs结合的重要性,这将有助于未来识别新型昆虫诱捕剂。

相似文献

1
Identification of the odorant binding proteins of Western Flower Thrips (), characterization and binding analysis of with molecular modelling, molecular dynamics simulations and a confirmatory field trial.西花蓟马气味结合蛋白的鉴定、通过分子建模、分子动力学模拟及验证性田间试验对其进行表征和结合分析。
J Biomol Struct Dyn. 2024 Feb 28:1-16. doi: 10.1080/07391102.2024.2317990.
2
Comparison and Functional Analysis of Odorant-Binding Proteins and Chemosensory Proteins in Two Closely Related Thrips Species, and (Thysanoptera: Thripidae) Based on Antennal Transcriptome Analysis.基于触角转录组分析比较两种近缘蓟马物种 和 (缨翅目:蓟马科)的气味结合蛋白和化学感受蛋白并进行功能分析。
Int J Mol Sci. 2022 Nov 11;23(22):13900. doi: 10.3390/ijms232213900.
3
Attraction of Females towards the Aggregation Pheromone Neryl ()-2-Methylbutanoate and Kairomones in a Y-Olfactometer.在Y型嗅觉仪中雌性对聚集信息素香叶基()-2-甲基丁酸酯及利它素的趋性
Insects. 2023 Jun 16;14(6):562. doi: 10.3390/insects14060562.
4
Molecular Characterization and Binding Properties of Odorant Binding Protein 7 in Western Flower Thrips, (Thysanoptera: Thripidae).西花蓟马(缨翅目:蓟马科)中气味结合蛋白7的分子特征及结合特性
J Agric Food Chem. 2025 Apr 2;73(13):7574-7583. doi: 10.1021/acs.jafc.4c10456. Epub 2025 Mar 20.
5
Selection Behavior and OBP-Transcription Response of Western Flower Thrips, , to Six Plant VOCs from Kidney Beans.西花蓟马对菜豆中 6 种植物挥发性有机化合物的选择行为及其 OBP-转录反应
Int J Mol Sci. 2023 Aug 14;24(16):12789. doi: 10.3390/ijms241612789.
6
A new attractant for monitoring western flower thrips, Frankliniella occidentalis in protected crops.一种用于监测保护地作物中西方花蓟马(Frankliniella occidentalis)的新型引诱剂。
Springerplus. 2015 Feb 24;4:89. doi: 10.1186/s40064-015-0864-3. eCollection 2015.
7
Molecular characterisation and expression profiles of an odorant-binding proteins gene (FoccOBP9) from .来自……的一种气味结合蛋白基因(FoccOBP9)的分子特征及表达谱
Bull Entomol Res. 2025 Feb;115(1):74-83. doi: 10.1017/S0007485324000683.
8
Semiochemicals Associated with the Western Flower Thrips Attraction: A Systematic Literature Review and Meta-Analysis.与西花蓟马吸引相关的信息化合物:系统文献综述与荟萃分析
Insects. 2023 Mar 8;14(3):269. doi: 10.3390/insects14030269.
9
RNA interference tools for the western flower thrips, Frankliniella occidentalis.用于西花蓟马(Frankliniella occidentalis)的RNA干扰工具。
J Insect Physiol. 2015 May;76:36-46. doi: 10.1016/j.jinsphys.2015.03.009. Epub 2015 Mar 18.
10
4-Pyridyl carbonyl compounds as thrips lures: effectiveness for Western flower thrips in y-tube bioassays.4-吡啶基羰基化合物作为蓟马引诱剂:在Y型管生物测定中对西花蓟马的有效性
J Agric Food Chem. 2008 Aug 13;56(15):6554-61. doi: 10.1021/jf800863t. Epub 2008 Jul 4.

引用本文的文献

1
Exploring Natural Compounds as Potential CDK4 Inhibitors for Therapeutic Intervention in Neurodegenerative Diseases through Computational Analysis.通过计算分析探索天然化合物作为潜在的CDK4抑制剂用于神经退行性疾病的治疗干预。
Mol Biotechnol. 2024 Aug 29. doi: 10.1007/s12033-024-01258-8.
2
Optimizing the resveratrol fragments for novel in silico hepatocellular carcinoma de novo drug design.优化白藜芦醇片段,用于新型计算机辅助肝细胞癌从头药物设计。
Sci Rep. 2024 Jul 28;14(1):17336. doi: 10.1038/s41598-024-68403-w.