Chappuis Charles J F, Cléroux Marilyn, Descombes Corentin, Barth Yannick, Lefort François
Analytical Chemistry Group, Haute École de Viticulture et Oenologie de Changins, HES-SO University of Applied Sciences and Arts Western Switzerland, 1260 Nyon, Switzerland.
Plants and Pathogens Group, Research Institute Land Nature Environment, Geneva School of Engineering Architecture and Landscape, HES-SO University of Applied Sciences and Arts Western Switzerland, 1254 Jussy, Switzerland.
Insects. 2023 Jun 16;14(6):562. doi: 10.3390/insects14060562.
An understanding of insect olfaction allows for more specific alternative methods of pest control. We evaluated the responses of the western flower thrips (WFT, ) in a Y-olfactometer to estimate gas-phase concentrations of the aggregation pheromone neryl ()-2-methylbutanoate and known kairomones such as methyl isonicotinate, ()-(-)-verbenone, and -anisaldehyde. The gas-phase concentrations of these compounds were obtained from the release rates measured in dynamic headspace cells. The compounds were collected from the headspace using dried solid-phase extraction (SPE) cartridges and analyzed with a triple quadrupole GC-MS/MS. We observed that the aggregation pheromone significantly attracted WFT females at doses of 10 and 100 µg, whereas methyl isonicotinate and -anisaldehyde significantly attracted WFT females at the highest dose. Verbenone did not produce any significant results. A completely different picture was obtained when the gas-phase concentrations were considered. The minimal gas-phase concentrations of the pheromone required to attract WFT females was 0.027 ng/mL, at least 100 times lower than that of the other two compounds. The relevance and implications of our results are discussed in light of the insect's biology and pest management methods.
对昆虫嗅觉的了解有助于采用更具针对性的害虫防治替代方法。我们在Y型嗅觉仪中评估了西花蓟马（WFT）对聚集信息素橙花叔醇（）-2-甲基丁酸酯以及已知的利它素如烟酸甲酯、（）-（-）-马鞭草烯酮和对甲氧基苯甲醛的气相浓度的反应。这些化合物的气相浓度是通过动态顶空细胞中测量的释放速率获得的。使用干燥的固相萃取（SPE）柱从顶空中收集这些化合物，并用三重四极杆气相色谱-质谱联用仪（GC-MS/MS）进行分析。我们观察到，聚集信息素在10和100微克剂量下能显著吸引西花蓟马雌虫，而烟酸甲酯和对甲氧基苯甲醛在最高剂量下能显著吸引西花蓟马雌虫。马鞭草烯酮未产生任何显著结果。当考虑气相浓度时，得到了完全不同的结果。吸引西花蓟马雌虫所需的信息素的最低气相浓度为0.027纳克/毫升, 至少比其他两种化合物低100倍。我们根据昆虫生物学和害虫管理方法讨论了研究结果的相关性和意义。
