Gong Yafei, Fu Yan, Lou Dawei
Department of Analytical Chemistry, Jilin Institute of Chemical Technology, Jilin, 132022, P.R. China.
J Fluoresc. 2025 Mar;35(3):1599-1609. doi: 10.1007/s10895-024-03633-9. Epub 2024 Feb 28.
Abnormal tryptophan (Trp) metabolism can be used as an important indicator of chronic hepatitis, paranoia, Parkinson's disease and other diseases. Deficiency or excessive accumulation of Cu can cause diseases such as Wilson's disease and Alzheimer's disease. Eu-based metal-organic framework (Eu-MOF) was successfully prepared for fluorescence sensing of Trp and Cu in an aqueous solution (pH = 7.4). Eu-MOF showed high selectivity and sensitivity for Trp and Cu with detection limits of 0.22 µM and 0.09 µM and K of 6.17 × 103 M and 2.37 × 10 M respectively. Trp and Cu2+ had overlapped UV absorption spectra with that of Eu-MOF and competed for the excitation light source. Trp also attenuated the antennae effect of organic ligands on Eu-MOF, thus quenching the red fluorescence of Eu-MOF. This study provides insights into the application of MOFs in bioanalysis and diagnostics.
异常的色氨酸（Trp）代谢可作为慢性肝炎、妄想症、帕金森病等疾病的重要指标。铜的缺乏或过度积累会引发诸如威尔逊氏病和阿尔茨海默病等疾病。成功制备了基于铕的金属有机框架（Eu-MOF）用于水溶液（pH = 7.4）中色氨酸和铜的荧光传感。Eu-MOF对色氨酸和铜表现出高选择性和灵敏度，检测限分别为0.22 µM和0.09 µM，K值分别为6.17×10³ M和2.37×10 M。色氨酸和Cu²⁺与Eu-MOF具有重叠的紫外吸收光谱，并竞争激发光源。色氨酸还减弱了有机配体对Eu-MOF的天线效应，从而淬灭了Eu-MOF的红色荧光。本研究为金属有机框架在生物分析和诊断中的应用提供了见解。
