Department of Health Sciences, Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Department of Physical Therapy, Postgraduate Program in Physiotherapy, Federal University of São Carlos, São Paulo, Brazil.
Photobiomodul Photomed Laser Surg. 2024 Mar;42(3):200-207. doi: 10.1089/photob.2023.0137. Epub 2024 Feb 28.
Investigating the effect of different parameters of photobiomodulation (PBM) with low-power laser on multi-potent mesenchymal stem cells (MSCs) derived from adipose tissue in terms of proliferation and cell death. MSCs were submitted to PBM applications with combinations of the following physical parameters: control group (no intervention), wavelengths of 660 and 830 nm; energy of 0.5, 2, and 4 J; and power of 40 and 100 mW. MSC analysis was performed using MetaXpress software at 24, 48, and 72 h. Irradiation promoted a significant increase in cell proliferation ( < 0.05), with 830 nm laser, 100 mW, with energy of 0.5, 2, and 4 J in relation to the control group at all times. PBM with 660 nm, power of 40 mW, and energy of 0.5, 2, and 4 J produced greater cell death at 24 h compared with the control group. At the time of 72 h, there was no significant difference concerning cell death. According to the results found, we can conclude that both wavelengths were effective; however, the 830 nm laser was more effective in terms of cell proliferation compared with the 660 nm laser. The 660 nm wavelength showed a significant increase in cell death when compared with the 830 nm laser.
研究不同低强度光生物调节（PBM）参数对脂肪来源多能间充质干细胞（MSCs）增殖和细胞死亡的影响。将 MSCs 暴露于以下物理参数组合的 PBM 应用中：对照组（无干预）、波长 660nm 和 830nm；能量 0.5、2 和 4J；功率 40 和 100mW。在 24、48 和 72h 使用 MetaXpress 软件对 MSC 进行分析。照射促进细胞增殖显著增加（<0.05），与对照组相比，830nm 激光、100mW、0.5、2 和 4J 能量在所有时间点均如此。与对照组相比，660nm、40mW 功率和 0.5、2 和 4J 能量在 24h 时产生更多的细胞死亡。在 72h 时，细胞死亡没有显著差异。根据发现的结果，我们可以得出结论，两种波长都有效；然而，830nm 激光在细胞增殖方面比 660nm 激光更有效。与 830nm 激光相比，660nm 波长的细胞死亡显著增加。
