Department of Gastrointestinal Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China.
Department of Gastrointestinal Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510080, China; Department of General Surgery, Guangdong Provincial People's Hospital Ganzhou Hospital (Ganzhou Municipal Hospital), Ganzhou, 341000, China.
Microbes Infect. 2024 May-Jun;26(4):105315. doi: 10.1016/j.micinf.2024.105315. Epub 2024 Feb 27.
The current study proposed a novel subtype, Human papillomavirus (HPV)-infected colorectal cancer (CRC), to understand the impact of HPV on CRC.
We assessed the prevalence and clinical implications of HPV in CRC by integrating a single cohort in Guangdong Provincial People's Hospital and public datasets. Differential gene, pathway enrichment, and immune infiltration analysis were conducted to explore the patterns in HPV-infected CRC. Quantitative polymerase chain reaction, cell proliferation, scratch, and flow cytometry assays were employed to validate the impact of HPV on CRC.
The study revealed a high prevalence of HPV infection in CRC, with infection rates ranging from 10% to 31%. There was also a significant increase in tumor proliferation in HPV-infected CRC. The study showed increased immune cell infiltration, including T cells, γδ T cells, cytotoxic cells, and plasmacytoid dendritic cells in HPV-infected CRC (P < 0.05). Furthermore, our findings confirmed that HPV infection promoted M1 polarization. Our results demonstrated that low ISM2 expression was associated with a less advanced clinical stage (P < 0.001) and better survival outcomes (P = 0.039). Low ISM2 expression correlated with a strong tumor immune response, potentially contributing to the improved survival observed in HPV-infected CRC.
These findings provided a novel subtype of HPV-infected CRC. The subtype with a better prognosis showed a "hot" tumor immune microenvironment that may be responsive to immunotherapy.
本研究提出了一种新的亚型，即人乳头瘤病毒（HPV）感染的结直肠癌（CRC），以了解 HPV 对 CRC 的影响。
我们通过整合广东省人民医院的一个单一队列和公共数据集来评估 HPV 在 CRC 中的流行率和临床意义。进行差异基因、通路富集和免疫浸润分析，以探讨 HPV 感染的 CRC 中的模式。通过定量聚合酶链反应、细胞增殖、划痕和流式细胞术实验来验证 HPV 对 CRC 的影响。
本研究显示 HPV 在 CRC 中的感染率很高，从 10%到 31%不等。HPV 感染的 CRC 中肿瘤增殖显著增加。研究表明 HPV 感染导致 CRC 中免疫细胞浸润增加，包括 T 细胞、γδ T 细胞、细胞毒性细胞和浆细胞样树突状细胞（P<0.05）。此外，我们的研究结果证实 HPV 感染促进了 M1 极化。我们的结果表明，低表达 ISM2 与较晚的临床分期（P<0.001）和更好的生存结果相关（P=0.039）。低 ISM2 表达与强烈的肿瘤免疫反应相关，这可能是 HPV 感染的 CRC 观察到的生存改善的原因之一。
这些发现提供了一种新的 HPV 感染的 CRC 亚型。预后较好的亚型表现出“热”肿瘤免疫微环境，可能对免疫治疗有反应。
