State Key Laboratory for Diagnosis and Treatment of Infectious Disease, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Colorectal Dis. 2011 Aug;13(8):865-71. doi: 10.1111/j.1463-1318.2010.02335.x. Epub 2010 May 28.
The prevalence of human papillomavirus (HPV) was determined in Chinese patients with colorectal cancer (CRC). The study also aimed to determine whether the HPV DNA peripheral blood (PB) assay can be used to diagnose HPV-related CRC.
Tumour tissue, noncancerous colorectal tissue and whole-blood samples were obtained from 96 patients with CRC. In addition, 32 colorectal tissue samples were harvested from patients without CRC, and 48 whole-blood samples were collected from healthy blood donors. HPV DNA was detected by means of a nested polymerase chain reaction (PCR) using consensus primers, and HPV genotypes were determined by reverse Southern blot and pyrosequencing.
HPV DNA was detected in 32 of the 96 patients with CRC, and colorectal tissues from the 32 control patients without CRC were negative for HPV DNA (P < 0.001). Among 48 healthy donors, three had detectable levels of HPV DNA in their PB. Patients with CRC did not have significantly higher levels of HPV DNA than controls. The HPV prevalence in tumour tissues was higher than that in noncancerous colorectal tissues (P < 0.001) or that in PB samples (P < 0.001). No correlation between the presence of HPV and demographic or medical characteristics was observed. HPV 16 was the viral type most frequently detected and was found in 33 (94%) of 35 HPV-positive patients.
HPV infection may be a risk factor for CRC. However, detection of HPV DNA in PB does not appear to reflect the HPV status of CRC.
确定人乳头瘤病毒（HPV）在中国结直肠癌（CRC）患者中的流行情况。本研究还旨在确定 HPV 外周血（PB）检测是否可用于诊断 HPV 相关性 CRC。
从 96 例 CRC 患者中获取肿瘤组织、非癌结直肠组织和全血样本。此外，从 32 例无 CRC 的患者中采集了 32 例结直肠组织样本，从 48 名健康献血者中采集了 48 例全血样本。采用巢式聚合酶链反应（PCR）用通用引物检测 HPV DNA，并通过反向 Southern 印迹和焦磷酸测序确定 HPV 基因型。
在 96 例 CRC 患者中有 32 例检测到 HPV DNA，而 32 例无 CRC 的对照患者的结直肠组织均未检测到 HPV DNA（P<0.001）。在 48 名健康供体中，有 3 名供体的 PB 中可检测到 HPV DNA。CRC 患者的 HPV DNA 水平与对照组相比无显著差异。肿瘤组织中的 HPV 患病率高于非癌结直肠组织（P<0.001）或 PB 样本（P<0.001）。未观察到 HPV 存在与人口统计学或医学特征之间存在相关性。HPV 16 是最常检测到的病毒类型，在 35 例 HPV 阳性患者中有 33 例（94%）存在 HPV 16。
HPV 感染可能是 CRC 的危险因素。然而，PB 中 HPV DNA 的检测似乎并不能反映 CRC 的 HPV 状态。
