使用双波长光声显微镜对房水静脉和周围巩膜进行三维成像。
3D imaging of aqueous veins and surrounding sclera using a dual-wavelength photoacoustic microscopy.
作者信息
Ni Linyu, Zhang Wei, Kim Wonsuk, Warchock Alexus, Bicket Amanda, Wang Xueding, Moroi Sayoko E, Argento Alan, Xu Guan
机构信息
Department of Ophthalmology and Visual Sciences, Department of Biomedical Engineering, University of Michigan, 500 S. State St., Ann Arbor, MI 48109, USA.
Department of Biomedical Engineering, University of Michigan, 500 S. State St., Ann Arbor, MI 48109, USA.
出版信息
Biomed Opt Express. 2023 Nov 16;14(12):6291-6300. doi: 10.1364/BOE.505288. eCollection 2023 Dec 1.
Understanding aqueous outflow resistance at the level of aqueous veins has been a challenge to the management of glaucoma. This study investigated resolving the anatomies of aqueous veins and the textures of surrounding sclera using photoacoustic microscopy (PAM). A dual wavelength PAM system was established and validated using imaging phantoms, porcine and human globes perfused with an optical contrast agent . The system shows lateral resolution of 8.23 µm and 4.70 µm at 1200 nm and 532 nm, respectively, and an axial resolution of 27.6 µm. The system is able to separately distinguish the aqueous veins and the sclera with high contrast in full circumference of the porcine and human globes.
了解巩膜静脉水平的房水流出阻力一直是青光眼治疗面临的挑战。本研究利用光声显微镜（PAM）研究巩膜静脉的解剖结构和周围巩膜的纹理。建立了双波长PAM系统，并使用成像体模、灌注光学造影剂的猪眼和人眼进行了验证。该系统在1200 nm和532 nm处的横向分辨率分别为8.23 µm和4.70 µm，轴向分辨率为27.6 µm。该系统能够在猪眼和人眼的整个圆周上以高对比度分别区分巩膜静脉和巩膜。
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