触觉需要机械门控离子通道ELKIN1。

Touch sensation requires the mechanically gated ion channel ELKIN1.

作者信息

Chakrabarti Sampurna, Klich Jasmin D, Khallaf Mohammed A, Hulme Amy J, Sánchez-Carranza Oscar, Baran Zuzanna M, Rossi Alice, Huang Angela Tzu-Lun, Pohl Tobias, Fleischer Raluca, Fürst Carina, Hammes Annette, Bégay Valérie, Hörnberg Hanna, Finol-Urdaneta Rocio K, Poole Kate, Dottori Mirella, Lewin Gary R

机构信息

Molecular Physiology of Somatic Sensation Laboratory, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin-Buch, Germany.

Department of Zoology and Entomology, Faculty of Science, Assiut University, Assiut 71516, Egypt.

出版信息

Science. 2024 Mar;383(6686):992-998. doi: 10.1126/science.adl0495. Epub 2024 Feb 29.

DOI:10.1126/science.adl0495

PMID:38422143

Abstract

Touch perception is enabled by mechanically activated ion channels, the opening of which excites cutaneous sensory endings to initiate sensation. In this study, we identify ELKIN1 as an ion channel likely gated by mechanical force, necessary for normal touch sensitivity in mice. Touch insensitivity in mice was caused by a loss of mechanically activated currents (MA currents) in around half of all sensory neurons activated by light touch (low-threshold mechanoreceptors). Reintroduction of into sensory neurons from mice restored MA currents. Additionally, small interfering RNA-mediated knockdown of from induced human sensory neurons substantially reduced indentation-induced MA currents, supporting a conserved role for ELKIN1 in human touch. Our data identify ELKIN1 as a core component of touch transduction in mice and potentially in humans.

摘要

触觉感知由机械激活离子通道实现，这些通道的开放会刺激皮肤感觉末梢以引发感觉。在本研究中，我们确定ELKIN1是一种可能受机械力门控的离子通道，它对小鼠的正常触觉敏感性至关重要。小鼠的触觉不敏感是由约一半受轻触激活的感觉神经元（低阈值机械感受器）中机械激活电流（MA电流）的丧失所致。将其重新引入来自[具体小鼠品系]小鼠的感觉神经元可恢复MA电流。此外，小干扰RNA介导的从诱导的人类感觉神经元中敲低[ELKIN1]可大幅降低压痕诱导的MA电流，这支持了ELKIN1在人类触觉中具有保守作用。我们的数据确定ELKIN1是小鼠触觉转导的核心成分，在人类中可能也是如此。