School of Food science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; School of Biological and Pharmaceutical Sciences, Shaanxi University of Science and Technology, Xi'an 710021, China.
School of Food science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; School of Biological and Pharmaceutical Sciences, Shaanxi University of Science and Technology, Xi'an 710021, China.
Int J Biol Macromol. 2024 Mar;262(Pt 1):129856. doi: 10.1016/j.ijbiomac.2024.129856. Epub 2024 Feb 29.
In this study, a novel polysaccharide, AAP-2S, was extracted from Auricularia auricula, and the anti-glycosylation effect of AAP-2S and its underlying mechanisms were investigated using an in vitro BSA-fructose model and a cellular model. The results demonstrated the inhibiting formation of advanced glycation end products (AGEs) in vitro by AAP-2S. Concurrently, it attenuated oxidative damage to proteins in the model, preserved protein sulfhydryl groups from oxidation, reduced protein carbonylation, prevented structural alterations in proteins, and decreased the formation of β-crosslinked structures. Furthermore, AAP-2S demonstrated metal-chelating capabilities. GC-MS/MS-based metabolomics were employed to analyze changes in metabolic profiles induced by AAP-2S in a CML-induced HK-2 cell model. Mechanistic investigations revealed that AAP-2S could mitigate glycosylation and ameliorate cell fibrosis by modulating the RAGE/TGF-β/NOX4 pathway. This study provides a foundational framework for further exploration of Auricularia auricular polysaccharide as a natural anti-AGEs agent, paving the way for its potential development and application as a food additive.
在这项研究中，从银耳中提取了一种新型多糖 AAP-2S，并使用体外 BSA-果糖模型和细胞模型研究了 AAP-2S 的抗糖化作用及其潜在机制。结果表明 AAP-2S 可抑制体外晚期糖基化终产物（AGEs）的形成。同时，它减轻了模型中蛋白质的氧化损伤，保护了蛋白质巯基不被氧化，减少了蛋白质羰基化，防止了蛋白质结构的改变，并减少了β-交联结构的形成。此外，AAP-2S 还具有金属螯合能力。基于 GC-MS/MS 的代谢组学分析了 AAP-2S 在 CML 诱导的 HK-2 细胞模型中引起的代谢谱变化。机制研究表明，AAP-2S 通过调节 RAGE/TGF-β/NOX4 通路减轻糖基化并改善细胞纤维化。这项研究为进一步探索银耳多糖作为天然抗 AGEs 剂提供了基础框架，为其作为食品添加剂的潜在开发和应用铺平了道路。
