Jiangsu Key laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agricultural and Rural Affairs, The Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
In Vitro Cell Dev Biol Anim. 2024 Mar;60(3):258-265. doi: 10.1007/s11626-024-00861-y. Epub 2024 Feb 29.
It has been demonstrated that angiopoietin-like protein 4 (ANGPTL4) plays an important regulatory role in lipid metabolism and backfat deposition appears to vary in different pig breeds. However, the correlation between ANGPTL4 and backfat deposition have not been well characterized and the role of ANGPTL4 in regulating adipogenesis remains unclear. Therefore, this study aimed to investigate correlation between ANGPTL4 and backfat deposition and to explore the effects of ANGPTL4 on preadipocyte differentiation and the underlying mechanism. Our results showed that the backfat thickness and the ANGPTL4 gene expression of Laiwu pigs were significantly higher than those in DLY pigs and the ANGPTL4 gene expression was positively correlated with backfat thickness both in DLY pigs and Laiwu pigs. Moreover, an increase in ANGPTL4 expression and activation of autophagy were observed during the differentiation of stromal vascular fraction cells. In addition, knockdown of ANGPTL4 inhibited the differentiation of 3T3-L1 cells with decreased expression of LC3-II and ATG5 and increased expression of SQSTM1, suggesting the involvement of autophagy in ANGPTL4-mediated adipogenesis. In conclusion, these results suggested that ANGPTL4 is positively correlated with backfat deposition in pigs and knockdown of ANGPTL4 inhibits adipogenesis of preadipocyte via autophagy, providing new insights into the regulation of fat deposition and to improve the carcass quality and meat quality of porcine.
已经证明，血管生成素样蛋白 4（ANGPTL4）在脂质代谢中发挥重要的调节作用，背膘厚度似乎在不同猪品种中存在差异。然而，ANGPTL4 与背膘沉积之间的相关性尚未得到很好的描述，ANGPTL4 在调节脂肪生成中的作用仍不清楚。因此，本研究旨在探讨 ANGPTL4 与背膘沉积的相关性，并研究 ANGPTL4 对前体脂肪细胞分化的影响及其潜在机制。
我们的研究结果表明，莱芜猪的背膘厚度和 ANGPTL4 基因表达显著高于 DLY 猪，并且 DLY 猪和莱芜猪的 ANGPTL4 基因表达与背膘厚度呈正相关。此外，在基质血管成分细胞的分化过程中观察到 ANGPTL4 表达增加和自噬激活。此外，ANGPTL4 的敲低抑制了 3T3-L1 细胞的分化，LC3-II 和 ATG5 的表达降低，SQSTM1 的表达增加，表明自噬参与了 ANGPTL4 介导的脂肪生成。
综上所述，这些结果表明，ANGPTL4 与猪的背膘沉积呈正相关，敲低 ANGPTL4 通过自噬抑制前体脂肪细胞的脂肪生成，为脂肪沉积的调节提供了新的见解，并改善了猪的胴体品质和肉质。
