Cytologic diagnosis of the acute nonlymphoid leukemias. II. Flow cytometry, surface markers, cytogenetics, and use of cell culture techniques.

Part I of this two-part article discussed the use of morphologic, histochemical, and ultrastructural studies for the diagnosis and classification of ANLL variants of acute nonlymphoid leukemia. However, a small proportion of acute leukemias are not amenable to definition by these techniques and have, in the past, been classified as acute undifferentiated leukemias. The use of supplemental techniques such as flow cytometry, surface marker analysis, cytogenetics, and in vitro growth patterns will often identify the correct cellular lineage for these cases.