Department of Neurosurgery, Center for Neuroregeneration, Houston Methodist Academic Institute, Houston, TX, USA.
Methods Mol Biol. 2024;2761:589-597. doi: 10.1007/978-1-0716-3662-6_39.
Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO) (Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M, Cell 159:896-910, 2014) aims to match the refractive index (RI) of tissue to the surrounding medium, thereby facilitating three-dimensional (3D) imaging and quantification of cellular points and tissue structures. Once cleared, transparent tissue samples allow for rapid imaging with no mechanical sectioning. This imaging technology enables us to visualize brain tissue in situ and quantify the morphology and extent of glial cell branches or neuronal processes extending from the epicenter of a traumatic brain injury (TBI). In this way, we can more accurately assess and quantify the damaging consequences of TBI not only in the impact region but also in the extended pericontusional regions.
免疫标记增强的组织透明化成像（iDISCO）（Renier N、Wu Z、Simon DJ、Yang J、Ariel P、Tessier-Lavigne M，Cell 159:896-910, 2014）旨在匹配组织的折射率（RI）与周围介质，从而促进细胞点和组织结构的三维（3D）成像和定量。一旦被清除，透明的组织样本允许快速成像，而无需进行机械切片。这种成像技术使我们能够对脑组织进行原位可视化，并定量分析从创伤性脑损伤（TBI）中心延伸出的神经胶质细胞分支或神经元突起的形态和范围。通过这种方式，我们不仅可以在撞击区域，而且可以在扩展的脑损伤周边区域，更准确地评估和量化 TBI 的破坏性后果。
